Near-Atomic Resolution Structure Determination in Over-Focus with Volta   Phase Plate by Cs-Corrected Cryo-EM

doi:10.1016/j.str.2017.08.008

CORC > 北京大学 > 化学与分子工程学院

	Near-Atomic Resolution Structure Determination in Over-Focus with Volta Phase Plate by Cs-Corrected Cryo-EM
	Fan, Xiao ; Zhao, Lingyun ; Liu, Chuan ; Zhang, Jin-Can ; Fan, Kelong ; Yan, Xiyun ; Peng, Hai-Lin ; Lei, Jianlin ; Wang, Hong-Wei
刊名	STRUCTURE
	2017
关键词	CONTRAST CRYOELECTRON TOMOGRAPHY TRANSMISSION ELECTRON-MICROSCOPY BEAM-INDUCED MOTION PROTEIN COMPLEX H-FERRITIN VISUALIZATION NANOPARTICLES MICROGRAPHS DETECTORS RIBOSOME
DOI	10.1016/j.str.2017.08.008
英文摘要	Volta phase plate (VPP) is a recently developed transmission electron microscope (TEM) apparatus that can significantly enhance the image contrast of biological samples in cryoelectron microscopy, and therefore provide the possibility to solve structures of relatively small macromolecules at high-resolution. In this work, we performed theoretical analysis and found that using phase plate on objective lens spherical aberration (Cs)-corrected TEM may gain some interesting optical properties, including the over-focus imaging of macromolecules. We subsequently evaluated the imaging strategy of frozen-hydrated apo-ferritin with VPP on a Cs-corrected TEM and obtained the structure of apo-ferritin at near-atomic resolution from both under-and over-focused dataset, illustrating the feasibility and new potential of combining VPP with Cs-corrected TEM for high-resolution cryo-EM.; Ministry of Science and Technology of China [2016YFA0501100]; Beijing Municipal Science & Technology Commission [Z161100000116034]; CAST; Beijing Natural Science Foundation [5164037]; China Postdoctoral Science Foundation [2015M570158]; China Postdoctoral Science Special Foundation [2016T90143]; SCI(E); ARTICLE; 10; 1623-+; 25
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/470515]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Fan, Xiao,Zhao, Lingyun,Liu, Chuan,et al. Near-Atomic Resolution Structure Determination in Over-Focus with Volta Phase Plate by Cs-Corrected Cryo-EM[J]. STRUCTURE,2017.
APA	Fan, Xiao.,Zhao, Lingyun.,Liu, Chuan.,Zhang, Jin-Can.,Fan, Kelong.,...&Wang, Hong-Wei.(2017).Near-Atomic Resolution Structure Determination in Over-Focus with Volta Phase Plate by Cs-Corrected Cryo-EM.STRUCTURE.
MLA	Fan, Xiao,et al."Near-Atomic Resolution Structure Determination in Over-Focus with Volta Phase Plate by Cs-Corrected Cryo-EM".STRUCTURE (2017).