Ratiometric Fluorescent Biosensing of Hydrogen Peroxide and Hydroxyl   Radical in Living Cells with Lysozyme-Silver Nanoclusters: Lysozyme as   Stabilizing Ligand and Fluorescence Signal Unit

doi:10.1021/acs.analchem.6b02995

CORC > 北京大学 > 化学与分子工程学院

	Ratiometric Fluorescent Biosensing of Hydrogen Peroxide and Hydroxyl Radical in Living Cells with Lysozyme-Silver Nanoclusters: Lysozyme as Stabilizing Ligand and Fluorescence Signal Unit
	Liu, Fang ; Bing, Tao ; Shangguan, Dihua ; Zhao, Meiping ; Shao, Na
刊名	ANALYTICAL CHEMISTRY
	2016
关键词	PROTECTED GOLD NANOCLUSTERS PROTEIN-DIRECTED SYNTHESIS FENTON REACTION AMPEROMETRIC DETECTION SENSITIVE DETECTION AQUEOUS-SOLUTION FACILE SYNTHESIS QUANTUM DOTS GLUCOSE NANOPARTICLES
DOI	10.1021/acs.analchem.6b02995
英文摘要	Construction of facile ratiometric fluorescent probes which possess sensitive and selective sensing ability for bioactive small molecules is highly desirable and challenging. Herein, silver nanoclusters capped with denatured lysozyme (dLys-AgNCs) were synthesized and proved to be dual emissive. The facility of the dLys-AgNCs ratiometric probe was attributed to the finding that the lysozyme acted not only as stabilizing ligand but also as fluorescence signal unit. In the presence of Fenton reagents, the emission of dLys-AgNCs at 640 nm was quenched by center dot OH, whereas the emission at 450 was enhanced due to center dot OH-induced oxidation of tyrosine in the lysozyme. This probe could be used for highly sensitive detection of H2O2. The fluorescence changes of F450/F640 had fantastic linearity to H2O2 concentrations in the range of 0.8-200 mu mol/L (R2 = 0.9993), with a limit of detection (LOD) as low as 0.2 mu mol/L. Additionally, this probe was also applied to H2O2-generated oxidase-based biosensing. As a proof-of-concept, glucose and acetylcholine chloride were detected with benefical LOD values of 0.6 mu mol/L and 0.8 mu mol/L, respectively. Furthermore, fluorescence confocal imaging demonstrated dLys-AgNCs had a sensitive response to fluctuation of center dot OH levels in living cells, which might have promising application in study of center dot OH-induced oxidative damage to proteins.; National Natural Science Foundation of China [20905008]; Fundamental Research Funds for the Central Universities; SCI(E); PubMed; ARTICLE; shaona@bnu.edu.cn; 21; 10631-10638; 88
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/457180]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Liu, Fang,Bing, Tao,Shangguan, Dihua,et al. Ratiometric Fluorescent Biosensing of Hydrogen Peroxide and Hydroxyl Radical in Living Cells with Lysozyme-Silver Nanoclusters: Lysozyme as Stabilizing Ligand and Fluorescence Signal Unit[J]. ANALYTICAL CHEMISTRY,2016.
APA	Liu, Fang,Bing, Tao,Shangguan, Dihua,Zhao, Meiping,&Shao, Na.(2016).Ratiometric Fluorescent Biosensing of Hydrogen Peroxide and Hydroxyl Radical in Living Cells with Lysozyme-Silver Nanoclusters: Lysozyme as Stabilizing Ligand and Fluorescence Signal Unit.ANALYTICAL CHEMISTRY.
MLA	Liu, Fang,et al."Ratiometric Fluorescent Biosensing of Hydrogen Peroxide and Hydroxyl Radical in Living Cells with Lysozyme-Silver Nanoclusters: Lysozyme as Stabilizing Ligand and Fluorescence Signal Unit".ANALYTICAL CHEMISTRY (2016).