A fluorescence anisotropy study of the DNA hybridization reaction   mediated by formation of the C-Ag+-C structure

doi:10.1039/c6ay00459h

CORC > 北京大学 > 化学与分子工程学院

	A fluorescence anisotropy study of the DNA hybridization reaction mediated by formation of the C-Ag+-C structure
	Hong, Xinying ; Huang, Hongduan ; Chen, Mingxing ; Liu, Feng ; Li, Na
刊名	ANALYTICAL METHODS
	2016
关键词	MACROMOLECULE-LIGAND BINDING I-MOTIF DNA BASE-PAIRS SENSITIVE DETECTION MERCURY(II) IONS DUPLEX DNA POLARIZATION SILVER(I) APTAMER OCHRATOXIN
DOI	10.1039/c6ay00459h
英文摘要	Fluorescence anisotropy is a simple and cost-effective method which can be performed in a homogeneous manner, providing simplicity for practical usage. As it originates from the rotational diffusion of the fluorophore associated entity, this method is sensitive to the volume and structural changes of the investigated molecule. In this contribution, fluorescence anisotropy was used to study the DNA hybridization mediated by the formation of the C-Ag+-C structure with silver ions at the nano-molar level. Factors influencing the structural stability of the formed duplex, including buffer composition, the length of the formed duplex, and percentage of C-C mismatches, were studied in detail. The reagent background signal can be reduced by using a hairpin-structured, partially complementary strand with C-C mismatched sites. Fluorescence anisotropy titration was used to estimate the formation constant of the C-Ag+-C structure involving DNA hybridization based on a 1 : 1 binding stoichiometry between Ag+ and the C-C mismatched site. A good fit to the Scatchard model indicated that each binding site interacted independently with Ag+. This study demonstrated that fluorescence anisotropy is a simple and effective approach to study the structural stability of the short DNA molecule, thus extending the breadth of applications of fluorescence anisotropy in DNA-based recognition.; National Natural Science Foundation of China [21475004, 21035005, 21275011]; SCI(E); EI; ARTICLE; lina@pku.edu.cn; 15; 3156-3162; 8
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/437382]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Hong, Xinying,Huang, Hongduan,Chen, Mingxing,et al. A fluorescence anisotropy study of the DNA hybridization reaction mediated by formation of the C-Ag+-C structure[J]. ANALYTICAL METHODS,2016.
APA	Hong, Xinying,Huang, Hongduan,Chen, Mingxing,Liu, Feng,&Li, Na.(2016).A fluorescence anisotropy study of the DNA hybridization reaction mediated by formation of the C-Ag+-C structure.ANALYTICAL METHODS.
MLA	Hong, Xinying,et al."A fluorescence anisotropy study of the DNA hybridization reaction mediated by formation of the C-Ag+-C structure".ANALYTICAL METHODS (2016).