Highly sensitive detection of copper(I) and copper(II) in cell specimens   by CE-UV with large-volume sample stacking

doi:10.1039/c6ay00933f

CORC > 北京大学 > 化学与分子工程学院

	Highly sensitive detection of copper(I) and copper(II) in cell specimens by CE-UV with large-volume sample stacking
	Chen, Cheng ; Li, Xin ; Xie, Xia ; Chang, Fengxia ; Li, Meixian ; Zhu, Zhiwei
刊名	ANALYTICAL METHODS
	2016
关键词	SERUM COPPER CAPILLARY-ELECTROPHORESIS HODGKINS-DISEASE LYMPHOMAS LEUKEMIA COMPLEX ACID
DOI	10.1039/c6ay00933f
英文摘要	A sensitive, rapid and simple CE-UV method with large-volume sample stacking (LVSS) and polarity switching has been developed and validated for the analysis of copper(I) and copper(II) simultaneously. Benefiting from the combination of the LVSS method, a limit of detection (LOD) of 30 nM was obtained which was much lower than those of the current detection methods. Several factors, such as the concentration and pH of the background solution, injection time and pressure, stacking voltage and inversion electric current, were systematically optimized. Under the optimum conditions, the detection limits of copper(I) and copper(II) were improved nearly 1000 times compared with the conventional methods by CE-UV, which made it possible to determine trace copper in cell specimens. Eventually, the proposed method was successfully applied to detect copper(I) and copper(II) in spiked cell samples with good recoveries.; National Natural Science Foundation of China (NSFC) [21175006, 21335001, 21475002]; SCI(E); EI; ARTICLE; zwzhu@pku.edu.cn; 21; 4272-4276; 8
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/437145]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Chen, Cheng,Li, Xin,Xie, Xia,et al. Highly sensitive detection of copper(I) and copper(II) in cell specimens by CE-UV with large-volume sample stacking[J]. ANALYTICAL METHODS,2016.
APA	Chen, Cheng,Li, Xin,Xie, Xia,Chang, Fengxia,Li, Meixian,&Zhu, Zhiwei.(2016).Highly sensitive detection of copper(I) and copper(II) in cell specimens by CE-UV with large-volume sample stacking.ANALYTICAL METHODS.
MLA	Chen, Cheng,et al."Highly sensitive detection of copper(I) and copper(II) in cell specimens by CE-UV with large-volume sample stacking".ANALYTICAL METHODS (2016).