Dimensions, energetics, and denaturant effects of the protein   unstructured state

doi:10.1002/pro.2865

CORC > 北京大学 > 化学与分子工程学院

	Dimensions, energetics, and denaturant effects of the protein unstructured state
	Li, Maodong ; Liu, Zhirong
刊名	PROTEIN SCIENCE
	2016
关键词	protein denaturation single-molecule FRET transfer model binding model coil-globule transition SINGLE-MOLECULE SPECTROSCOPY INTRINSICALLY DISORDERED PROTEINS TEMPERATURE-INDUCED COLLAPSE COARSE-GRAINED MODELS UNFOLDED PROTEINS GUANIDINE-HYDROCHLORIDE NONNATIVE INTERACTIONS UREA TRANSITION THERMODYNAMICS
DOI	10.1002/pro.2865
英文摘要	Determining the energetics of the unfolded state of a protein is essential for understanding the folding mechanics of ordered proteins and the structure-function relation of intrinsically disordered proteins. Here, we adopt a coil-globule transition theory to develop a general scheme to extract interaction and free energy information from single-molecule fluorescence resonance energy transfer spectroscopy. By combining protein stability data, we have determined the free energy difference between the native state and the maximally collapsed denatured state in a number of systems, providing insight on the specific/nonspecific interactions in protein folding. Both the transfer and binding models of the denaturant effects are demonstrated to account for the revealed linear dependence of inter-residue interactions on the denaturant concentration, and are thus compatible under the coil-globule transition theory to further determine the dimension and free energy of the conformational ensemble of the unfolded state. The scaling behaviors and the effective -state are also discussed.; Ministry of Science and Technology of China [2015CB910300]; SCI(E); PubMed; ARTICLE; LiuZhiRong@pku.edu.cn; 3; 734-747; 25
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/435797]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Li, Maodong,Liu, Zhirong. Dimensions, energetics, and denaturant effects of the protein unstructured state[J]. PROTEIN SCIENCE,2016.
APA	Li, Maodong,&Liu, Zhirong.(2016).Dimensions, energetics, and denaturant effects of the protein unstructured state.PROTEIN SCIENCE.
MLA	Li, Maodong,et al."Dimensions, energetics, and denaturant effects of the protein unstructured state".PROTEIN SCIENCE (2016).