Lipidomic profiling of tryptophan hydroxylase 2 knockout mice reveals   novel lipid biomarkers associated with serotonin deficiency

doi:10.1007/s00216-015-9256-3

CORC > 北京大学 > 化学与分子工程学院

	Lipidomic profiling of tryptophan hydroxylase 2 knockout mice reveals novel lipid biomarkers associated with serotonin deficiency
	Weng, Rui ; Shen, Sensen ; Burton, Casey ; Yang, Li ; Nie, Honggang ; Tian, Yonglu ; Bai, Yu ; Liu, Huwei
刊名	ANALYTICAL AND BIOANALYTICAL CHEMISTRY
	2016
关键词	Serotonin deficiency Tryptophan hydroxylase 2 knockout (Tph2-/-) mice Lipidomics Lipid biomarkers Two-dimensional liquid chromatography-quadrupole time-of-flight mass spectrometry (2D LC-QToF-MS) FLIGHT MASS-SPECTROMETRY LC-QTOF-MS OXIDATIVE STRESS ALZHEIMERS-DISEASE BIOLOGICAL-MEMBRANES HUMAN PLASMA FATTY-ACIDS BRAIN CERAMIDE METABOLISM
DOI	10.1007/s00216-015-9256-3
英文摘要	Serotonin is an important neurotransmitter that regulates a wide range of physiological, neuropsychological, and behavioral processes. Consequently, serotonin deficiency is involved in a wide variety of neurodegenerative diseases, including Alzheimer's disease, Parkinson's disease, schizophrenia, and depression. The pathophysiological mechanisms underlying serotonin deficiency, particularly from a lipidomics perspective, remain poorly understood. This study therefore aimed to identify novel lipid biomarkers associated with serotonin deficiency by lipidomic profiling of tryptophan hydroxylase 2 knockout (Tph2-/-) mice. Using a high-throughput normal-/reversed-phase two-dimensional liquid chromatography-quadrupole time-of-flight mass spectrometry (NP/RP 2D LC-QToF-MS) method, 59 lipid biomarkers encompassing glycerophospholipids (glycerophosphocholines, lysoglycerophosphocholines, glycerophosphoethanolamines, lysoglycerophosphoethanolamines glycerophosphoinositols, and lysoglycerophosphoinositols), sphingolipids (sphingomyelins, ceramides, galactosylceramides, glucosylceramides, and lactosylceramides) and free fatty acids were identified. Systemic oxidative stress in the Tph2-/- mice was significantly elevated, and a corresponding mechanism that relates the lipidomic findings has been proposed. In summary, this work provides preliminary findings that lipid metabolism is implicated in serotonin deficiency.; National Natural Science Foundation of China [21322505, 21175008]; Ministry of Science and Technology of China [2012YQ09019409, 2013YQ510391]; National Science Foundation [DGE-1011744]; SCI(E); EI; PubMed; ARTICLE; yu.bai@pku.edu.cn; 11; 2963-2973; 408
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/435353]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Weng, Rui,Shen, Sensen,Burton, Casey,et al. Lipidomic profiling of tryptophan hydroxylase 2 knockout mice reveals novel lipid biomarkers associated with serotonin deficiency[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2016.
APA	Weng, Rui.,Shen, Sensen.,Burton, Casey.,Yang, Li.,Nie, Honggang.,...&Liu, Huwei.(2016).Lipidomic profiling of tryptophan hydroxylase 2 knockout mice reveals novel lipid biomarkers associated with serotonin deficiency.ANALYTICAL AND BIOANALYTICAL CHEMISTRY.
MLA	Weng, Rui,et al."Lipidomic profiling of tryptophan hydroxylase 2 knockout mice reveals novel lipid biomarkers associated with serotonin deficiency".ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2016).