Local de-condensation of double-stranded DNA in oppositely charged   polyelectrolyte as induced by spermidine

doi:10.1039/c5sm00552c

CORC > 北京大学 > 化学与分子工程学院

	Local de-condensation of double-stranded DNA in oppositely charged polyelectrolyte as induced by spermidine
	Pan, Wei ; Zhou, Jihan ; Yin, Yudan ; Wen, Hao ; Liang, Dehai
刊名	SOFT MATTER
	2015
关键词	MULTIVALENT SALT-SOLUTIONS REENTRANT CONDENSATION CHROMATIN FIBER POLYAMINES STABILITY POLYCATIONS COUNTERIONS TRANSITION DYNAMICS COLLAPSE
DOI	10.1039/c5sm00552c
英文摘要	How polyamines such as spermidine cooperate with histone to condense and de-condense DNA during transcription has not been clarified. In this work, using the complex of DNA and poly(L-lysine) (PLL) at +/- ratio of 0.5 as a model of nucleosome, we monitored the de-condensation of DNA in the presence of spermidine. As revealed by the results from atomic force microscopy and time-resolved laser light scattering, spermidine was able to transform the spherical complex into a core-shelled structure, with the hard core being the DNA-PLL complex and the soft shell being DNA and spermidine. The soft shell evolved into a coiled DNA conformation with time. Such a local de-condensation process should be helpful in understanding the DNA transcription and cell division process in vivo.; National Natural Science Foundation of China [21174007]; SCI(E); EI; PubMed; ARTICLE; dliang@pku.edu.cn; 23; 4705-4709; 11
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/420023]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Pan, Wei,Zhou, Jihan,Yin, Yudan,et al. Local de-condensation of double-stranded DNA in oppositely charged polyelectrolyte as induced by spermidine[J]. SOFT MATTER,2015.
APA	Pan, Wei,Zhou, Jihan,Yin, Yudan,Wen, Hao,&Liang, Dehai.(2015).Local de-condensation of double-stranded DNA in oppositely charged polyelectrolyte as induced by spermidine.SOFT MATTER.
MLA	Pan, Wei,et al."Local de-condensation of double-stranded DNA in oppositely charged polyelectrolyte as induced by spermidine".SOFT MATTER (2015).