No Significant Increase in the Delta 4-and Delta 7-Dafachronic Acid   Concentration in the Long-Lived glp-1 Mutant, nor in the Mutants   Defective in Dauer Formation

doi:10.1534/g3.115.018812

CORC > 北京大学 > 化学与分子工程学院

	No Significant Increase in the Delta 4-and Delta 7-Dafachronic Acid Concentration in the Long-Lived glp-1 Mutant, nor in the Mutants Defective in Dauer Formation
	Li, Tie-Mei ; Liu, Weilong ; Lu, Shan ; Zhang, Yan-Ping ; Jia, Le-Mei ; Chen, Jie ; Li, Xiangke ; Lei, Xiaoguang ; Dong, Meng-Qiu
刊名	G3-GENES GENOMES GENETICS
	2015
关键词	dafachronic acid steroid hormone glp-1 daf-12 C. elegans dauer NUCLEAR HORMONE-RECEPTOR C. ELEGANS DEVELOPMENT CAENORHABDITIS-ELEGANS LIFE-SPAN LONGEVITY SIGNALS QUANTIFICATION PLASTICITY DIAPAUSE LIGANDS
DOI	10.1534/g3.115.018812
英文摘要	The steroid hormone dafachronic acid (DA) regulates dauer formation and lifespan in Caenorhabditis elegans by binding to the nuclear receptor DAF-12. However, little is known about how DA concentrations change under various physiologic conditions and about how DA/DAF-12 signaling interacts with other signaling pathways that also regulate dauer formation and lifespan. Using a sensitive bioanalytical method, we quantified the endogenous DA concentrations in a long-lived germline-less glp-1 mutant and in the Dauer formation-defective (Daf-d) mutants daf-12, daf-16, daf-5, and daf-3. We found that the DA concentration in the glp-1 mutant was similar to that in the wild type (WT). This result is contrary to the long-held belief that germline loss-induced longevity involves increased DA production and suggests instead that this type of longevity involves an enhanced response to DA. We also found evidence suggesting that increased DA sensitivity underlies lifespan extension triggered by exogenous DA. At the L2/L3 stage, the DA concentration in a daf-12 null mutant decreased to 22% of the WT level. This finding is consistent with the previously proposed positive feedback regulation between DAF-12 and DA production. Surprisingly, the DA concentrations in the daf-16, daf-5, and daf-3 mutants were only 19-34% of theWT level at the L2/L3 stage, slightly greater than those in the Dauer formation-constitutive (Daf-c) mutants at the pre-dauer stage (4-15% of the WT L2 control). Our experimental evidence suggested that the positive feedback between DA and DAF-12 was partially induced in the three Daf-d mutants.; National Institutes of Health Office of Research Infrastructure Programs [P40 OD010440]; Ministry of Science and Technology of China [973-2012CB837400]; Ministry of Science and Technology of China (National Scientific Instrumentation Grant Program) [2011YQ09000506]; National Natural Science Foundation of China [21222209, 21472010, 91313303, 21375010]; municipal government of Beijing; SCI(E); ARTICLE; dongmengqiu@nibs.ac.cn; 7; 1473-1479; 5
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/418820]
专题	化学与分子工程学院生命科学学院
推荐引用方式 GB/T 7714	Li, Tie-Mei,Liu, Weilong,Lu, Shan,et al. No Significant Increase in the Delta 4-and Delta 7-Dafachronic Acid Concentration in the Long-Lived glp-1 Mutant, nor in the Mutants Defective in Dauer Formation[J]. G3-GENES GENOMES GENETICS,2015.
APA	Li, Tie-Mei.,Liu, Weilong.,Lu, Shan.,Zhang, Yan-Ping.,Jia, Le-Mei.,...&Dong, Meng-Qiu.(2015).No Significant Increase in the Delta 4-and Delta 7-Dafachronic Acid Concentration in the Long-Lived glp-1 Mutant, nor in the Mutants Defective in Dauer Formation.G3-GENES GENOMES GENETICS.
MLA	Li, Tie-Mei,et al."No Significant Increase in the Delta 4-and Delta 7-Dafachronic Acid Concentration in the Long-Lived glp-1 Mutant, nor in the Mutants Defective in Dauer Formation".G3-GENES GENOMES GENETICS (2015).