Chemical pulldown reveals dynamic pseudouridylation of the mammalian   transcriptome

doi:10.1038/NCHEMBIO.1836

CORC > 北京大学 > 化学与分子工程学院

	Chemical pulldown reveals dynamic pseudouridylation of the mammalian transcriptome
	Li, Xiaoyu ; Zhu, Ping ; Ma, Shiqing ; Song, Jinghui ; Bai, Jinyi ; Sun, Fangfang ; Yi, Chengqi
刊名	NATURE CHEMICAL BIOLOGY
	2015
关键词	MESSENGER-RNA NUCLEOTIDE RESOLUTION RIBOSOMAL-RNA HUMAN-CELLS SYNTHASE YEAST METHYLATION RESIDUES GENE IDENTIFICATION
DOI	10.1038/NCHEMBIO.1836
英文摘要	Pseudouridine (psi) is the most abundant post-transcriptional RNA modification, yet little is known about its prevalence, mechanism and function in mRNA. Here, we performed quantitative MS analysis and show that psi is much more prevalent (psi/U ratio similar to 0.2-0.6%) in mammalian mRNA than previously believed. We developed N-3-CMC-enriched pseudouridine sequencing (CeU-Seq), a selective chemical labeling and pulldown method, to identify 2,084 psi sites within 1,929 human transcripts, of which four (in ribosomal RNA and EEF1A1 mRNA) are biochemically verified. We show that hPUS1, a known psi synthase, acts on human mRNA; under stress, CeU-Seq demonstrates inducible and stress-specific mRNA pseudouridylation. Applying CeU-Seq to the mouse transcriptome revealed conserved and tissue-specific pseudouridylation. Collectively, our approaches allow comprehensive analysis of transcriptome-wide pseudouridylation and provide tools for functional studies of psi-mediated epigenetic regulation.; National Basic Research Foundation of China [2014CB964900]; National Natural Science Foundation of China [21472009, 31270838]; SCI(E); PubMed; ARTICLE; chengqi.yi@pku.edu.cn; 8; 592-U93; 11
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/417866]
专题	化学与分子工程学院生命科学学院
推荐引用方式 GB/T 7714	Li, Xiaoyu,Zhu, Ping,Ma, Shiqing,et al. Chemical pulldown reveals dynamic pseudouridylation of the mammalian transcriptome[J]. NATURE CHEMICAL BIOLOGY,2015.
APA	Li, Xiaoyu.,Zhu, Ping.,Ma, Shiqing.,Song, Jinghui.,Bai, Jinyi.,...&Yi, Chengqi.(2015).Chemical pulldown reveals dynamic pseudouridylation of the mammalian transcriptome.NATURE CHEMICAL BIOLOGY.
MLA	Li, Xiaoyu,et al."Chemical pulldown reveals dynamic pseudouridylation of the mammalian transcriptome".NATURE CHEMICAL BIOLOGY (2015).