Selective spectrophotometric determination of human serum albumin with   tetraiodo phenol sulfonphthalein

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	Selective spectrophotometric determination of human serum albumin with tetraiodo phenol sulfonphthalein
	Ma, CQ ; Li, KA ; Tong, SY
刊名	analytical letters
	1997
关键词	spectrophotometry protein tetraiodo phenol sulfonphthalein DYE-BINDING PROTEINS BLUE ASSAY
英文摘要	Tetraiodo phenol sulfonphthalein (TIPSP) reacts with protein rapidly to form the dye-protein complex with an absorbance maximum at 617 nm. Water is used as the blank instead of the reagent blank because the reagents have no absorption at this wavelength; thus the operating steps are simplified. Under the experimental conditions (0.03 mM TIPSP, pH 2.63), the linear range of this assay is composed of three parts: 10.2-54.4 mu g . ml(-1), 54.4-95.2 mu g . ml(-1) and 95.2-136.0 mu g . ml(-1). Most amino acids, reducing reagents (e.g. ascorbic acid) and complexing reagents (e.g. EDTA) don't interfere with the assay. The interferences from detergents, salts and ethanol can be minimized by dilution. In detection of human serum albumin, this method gave results close to that of the bromocresol green assay.; Chemistry, Analytical; SCI(E); 4; ARTICLE; 4; 739-752; 30
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/401928]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Ma, CQ,Li, KA,Tong, SY. Selective spectrophotometric determination of human serum albumin with tetraiodo phenol sulfonphthalein[J]. analytical letters,1997.
APA	Ma, CQ,Li, KA,&Tong, SY.(1997).Selective spectrophotometric determination of human serum albumin with tetraiodo phenol sulfonphthalein.analytical letters.
MLA	Ma, CQ,et al."Selective spectrophotometric determination of human serum albumin with tetraiodo phenol sulfonphthalein".analytical letters (1997).