Development of miniaturized competitive immunoassays on a protein chip   as a screening tool for drugs

doi:10.1373/clinchem.2004.036665

CORC > 北京大学 > 化学与分子工程学院

	Development of miniaturized competitive immunoassays on a protein chip as a screening tool for drugs
	Du, HW ; Wu, MT ; Yang, WP ; Yuan, G ; Sun, YM ; Lu, Y ; Zhao, S ; Du, QY ; Wang, F ; Yan, S ; Pan, M ; Lu, Y ; Wang, S ; Cheng, J
刊名	clinical chemistry
	2005
关键词	ANTIBODY MICROARRAY SPORT OLYMPICS FUTURE ABUSE
DOI	10.1373/clinchem.2004.036665
英文摘要	Background: Doping in sports has become a serious problem. Gas chromatography-mass spectrometry (GC-MS) serves as an effective reference method, but it is limited by low throughput and is therefore not suitable for large-scale screening. Use of protein chips for high-throughput screening of all athletes for prohibited substances could become an important complementary tool to GC-MS. Methods: We developed a protein chip based on an aldehyde-activated glass slide containing 10 physically isolated arrays. The chip was used to screen urine from 1347 athletes for prohibited substances and to screen a negative control group consisting of 200 females and 120 males. Urine samples from 66 individuals known to be abusers, provided by the China Doping Control Center (CDCC), and 129 standard prohibited substances were tested as positive controls. Results: All 1347 urine samples screened by means of the protein chips were also subjected to reference analysis by GC-MS at the CDCC. There was no qualitative difference between the results obtained with the two methods. The correlation coefficient (r(2)) for the quantitative results obtained with the protein chip and GC-MS was 0.991. Conclusions: The protein chip could be used to screen for a series of 16 prohibited drugs in urine samples. This system has the potential to become an effective screening method to test substances prohibited by the International Olympic Committee. (C) 2005 American Association for Clinical Chemistry.; Medical Laboratory Technology; SCI(E); 19; ARTICLE; 2; 368-375; 51
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/399913]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Du, HW,Wu, MT,Yang, WP,et al. Development of miniaturized competitive immunoassays on a protein chip as a screening tool for drugs[J]. clinical chemistry,2005.
APA	Du, HW.,Wu, MT.,Yang, WP.,Yuan, G.,Sun, YM.,...&Cheng, J.(2005).Development of miniaturized competitive immunoassays on a protein chip as a screening tool for drugs.clinical chemistry.
MLA	Du, HW,et al."Development of miniaturized competitive immunoassays on a protein chip as a screening tool for drugs".clinical chemistry (2005).