Increasing fatty acid production in E. coli by simulating the lipid accumulation of oleaginous microorganisms

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 材料生物技术研究中心

	Increasing fatty acid production in E. coli by simulating the lipid accumulation of oleaginous microorganisms
	Meng, Xin 1; Yang, Jianming 1; Cao, Yujin 1; Li, Liangzhi 1; Jiang, Xinglin 1; Xu, Xin 1; Liu, Wei 1; Xian, Mo1 ; Zhang, Yingwei 2
刊名	JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
	2011-08-01
卷号	38 期号:8 页码:919-925
关键词	Fatty acid Lipids Oleaginous microorganisms ACC NADP ME
ISSN号	1367-5435
中文摘要	Unlike many oleaginous microorganisms, E. coli only maintains a small amount of natural lipids in cells, impeding its utility to overproduce fatty acids. In this study, acetyl-CoA carboxylase (ACC) from Acinetobacter calcoaceticus was expressed in E. coli to redirect the carbon flux to the generation of malonyl-CoA, which resulted in a threefold increase in intracellular lipids. Moreover, providing a high level of NADPH by overexpressing malic enzyme and adding malate to the culture medium resulted in a fourfold increase in intracellular lipids (about 197.74 mg/g). Co-expression of ACC and malic enzyme resulted in 284.56 mg/g intracellular lipids, a 5.6-fold increase compared to the wild-type strain. This study provides some attractive strategies for increasing lipid production in E. coli by simulating the lipid accumulation of oleaginous microorganisms, which could aid the development of a prokaryotic fatty acid producer.
英文摘要	Unlike many oleaginous microorganisms, E. coli only maintains a small amount of natural lipids in cells, impeding its utility to overproduce fatty acids. In this study, acetyl-CoA carboxylase (ACC) from Acinetobacter calcoaceticus was expressed in E. coli to redirect the carbon flux to the generation of malonyl-CoA, which resulted in a threefold increase in intracellular lipids. Moreover, providing a high level of NADPH by overexpressing malic enzyme and adding malate to the culture medium resulted in a fourfold increase in intracellular lipids (about 197.74 mg/g). Co-expression of ACC and malic enzyme resulted in 284.56 mg/g intracellular lipids, a 5.6-fold increase compared to the wild-type strain. This study provides some attractive strategies for increasing lipid production in E. coli by simulating the lipid accumulation of oleaginous microorganisms, which could aid the development of a prokaryotic fatty acid producer.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	COENZYME-A CARBOXYLASE ; CELL OIL PRODUCTION ; ESCHERICHIA-COLI ; MALIC ENZYME ; ACETYL-COA ; MUCOR-CIRCINELLOIDES ; OVERPRODUCTION ; BIOSYNTHESIS ; BIODIESEL ; MICROBES
收录类别	SCI
语种	英语
WOS记录号	WOS:000293002000005
公开日期	2011-09-13
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn//handle/337004/678]
专题	青岛生物能源与过程研究所_材料生物技术研究中心
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuel, Qingdao, Peoples R China 2.Risun Coal Chem Grp Ltd, Xingtai, Peoples R China
推荐引用方式 GB/T 7714	Meng, Xin,Yang, Jianming,Cao, Yujin,et al. Increasing fatty acid production in E. coli by simulating the lipid accumulation of oleaginous microorganisms[J]. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,2011,38(8):919-925.
APA	Meng, Xin.,Yang, Jianming.,Cao, Yujin.,Li, Liangzhi.,Jiang, Xinglin.,...&Zhang, Yingwei.(2011).Increasing fatty acid production in E. coli by simulating the lipid accumulation of oleaginous microorganisms.JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,38(8),919-925.
MLA	Meng, Xin,et al."Increasing fatty acid production in E. coli by simulating the lipid accumulation of oleaginous microorganisms".JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 38.8(2011):919-925.