Enhancement of enzymatic in situ saccharification of cellulose in aqueous-ionic liquid media by ultrasonic intensification

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 材料生物技术研究中心

	Enhancement of enzymatic in situ saccharification of cellulose in aqueous-ionic liquid media by ultrasonic intensification
	Yang, Fang; Li, Liangzhi; Li, Qiang; Tan, Weigiang; Liu, Wei; Xian, Mo
刊名	CARBOHYDRATE POLYMERS
	2010-06-11
卷号	81 期号:2 页码:311-316
关键词	Cellulose Enzymatic hydrolysis In situ saccharification Ionic liquid Pretreatment Ultrasonic
ISSN号	0144-8617
中文摘要	A new approach for in situ enzymatic saccharification of cellulose in ionic liquids (ILs)-aqueous media is presented in which ultrasonic pretreatment was used to enhance the conversion of cellulose. For this purpose, the solubility of cellulose and the activity of cellulase were investigated in six alkylphosphate ILs. 1-Methyl-3-methylimidazolium dimethylphosphate ([Mmim][DMP]) giving favorable solubility and biocompatibility was selected to establish aqueous-ILs system for enzymatic in situ saccharification of cellulose. After further optimization of reaction parameters concerning cellulase concentration, temperature and IL concentration, higher conversion (95.48%) of cellulose was obtained in the media of aqueous-[Mmim][DMP] by conducting the pretreatment of cellulose with ultrasonic heating, whereas the conversion of cellulose untreated was 42.77%. Scanning electronic microscopy (SEM) and viscosity analysis indicated that IL-treated cellulose under ultrasonic condition was subjected to depolymerizadon, which led to more efficient saccharification. The findings of this study would have great implications for developing a continuous process for transformation of biomass such as straw cellulose to ethanol or other hydrocarbons. (C) 2010 Elsevier Ltd. All rights reserved.
英文摘要	A new approach for in situ enzymatic saccharification of cellulose in ionic liquids (ILs)-aqueous media is presented in which ultrasonic pretreatment was used to enhance the conversion of cellulose. For this purpose, the solubility of cellulose and the activity of cellulase were investigated in six alkylphosphate ILs. 1-Methyl-3-methylimidazolium dimethylphosphate ([Mmim][DMP]) giving favorable solubility and biocompatibility was selected to establish aqueous-ILs system for enzymatic in situ saccharification of cellulose. After further optimization of reaction parameters concerning cellulase concentration, temperature and IL concentration, higher conversion (95.48%) of cellulose was obtained in the media of aqueous-[Mmim][DMP] by conducting the pretreatment of cellulose with ultrasonic heating, whereas the conversion of cellulose untreated was 42.77%. Scanning electronic microscopy (SEM) and viscosity analysis indicated that IL-treated cellulose under ultrasonic condition was subjected to depolymerizadon, which led to more efficient saccharification. The findings of this study would have great implications for developing a continuous process for transformation of biomass such as straw cellulose to ethanol or other hydrocarbons. (C) 2010 Elsevier Ltd. All rights reserved.
学科主题	生物基化学品
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Applied ; Chemistry, Organic ; Polymer Science
研究领域[WOS]	Chemistry ; Polymer Science
关键词[WOS]	DISSOLUTION ; HYDROLYSIS ; PRETREATMENT ; BIOCATALYSIS ; MECHANISM ; ENZYMES
收录类别	SCI
语种	英语
WOS记录号	WOS:000278170400020
公开日期	2011-09-13
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn//handle/337004/670]
专题	青岛生物能源与过程研究所_材料生物技术研究中心
作者单位	Chinese Acad Sci, Key Lab Biofuel, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266061, Peoples R China
推荐引用方式 GB/T 7714	Yang, Fang,Li, Liangzhi,Li, Qiang,et al. Enhancement of enzymatic in situ saccharification of cellulose in aqueous-ionic liquid media by ultrasonic intensification[J]. CARBOHYDRATE POLYMERS,2010,81(2):311-316.
APA	Yang, Fang,Li, Liangzhi,Li, Qiang,Tan, Weigiang,Liu, Wei,&Xian, Mo.(2010).Enhancement of enzymatic in situ saccharification of cellulose in aqueous-ionic liquid media by ultrasonic intensification.CARBOHYDRATE POLYMERS,81(2),311-316.
MLA	Yang, Fang,et al."Enhancement of enzymatic in situ saccharification of cellulose in aqueous-ionic liquid media by ultrasonic intensification".CARBOHYDRATE POLYMERS 81.2(2010):311-316.