Production and characterization of monoclonal antibody against   recombinant human erythropoietin

CORC > 北京大学 > 化学与分子工程学院

	Production and characterization of monoclonal antibody against recombinant human erythropoietin
	Mi, Jie-Bo ; Yan, Jin ; Ding, Xia-Jie ; Guo, Zhen-Quan ; Zhao, Mei-Ping ; Chang, Wen-Bao
刊名	生物医学与环境科学英文版
	2007
关键词	recombinant human erythropoietin monoclonal antibody IgM ELISA
英文摘要	Objective To produce specific monoclonal antibody (mAb) against recombinant human erythropoietin (rHuEPO) for development of highly efficient methods for erythropoietin detection in biological fluids. Methods rHuEPO was covalently coupled with bovine serum albumin (BSA) and the conjugate was used to immunize mice to produce specific mAb against rHuEPO based on hybridoma technology. The obtained F-3-mAb was characterized by enzyme-linked immunosorbent assay (ELISA), SDS-PAGE and Western blot. Results The isotype of F-3-mAb was found to be IgM with an affinity constant of 2.1x10(8) L/mol. The competitive ELISA using the obtained IgM showed a broader linear range and lower detection limit compared with previous work. Conclusions The modification of rHuEPO was proved to be successful in generating required specific mAb with high avidity to rHuEPO.; Environmental Sciences; Public, Environmental & Occupational Health; SCI(E); 0; 3; 184-188; 20
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/397915]
专题	化学与分子工程学院生命科学学院
推荐引用方式 GB/T 7714	Mi, Jie-Bo,Yan, Jin,Ding, Xia-Jie,et al. Production and characterization of monoclonal antibody against recombinant human erythropoietin[J]. 生物医学与环境科学英文版,2007.
APA	Mi, Jie-Bo,Yan, Jin,Ding, Xia-Jie,Guo, Zhen-Quan,Zhao, Mei-Ping,&Chang, Wen-Bao.(2007).Production and characterization of monoclonal antibody against recombinant human erythropoietin.生物医学与环境科学英文版.
MLA	Mi, Jie-Bo,et al."Production and characterization of monoclonal antibody against recombinant human erythropoietin".生物医学与环境科学英文版 (2007).