Increasing unsaturated fatty acid contents in Escherichia coli by coexpression of three different genes

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 材料生物技术研究中心

	Increasing unsaturated fatty acid contents in Escherichia coli by coexpression of three different genes
	Cao, Yujin; Yang, Jianming; Xian, Mo; Xu, Xin; Liu, Wei
刊名	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
	2010-06-01
卷号	87 期号:1 页码:271-280
关键词	AtFatA Biodiesel fabA fabB Unsaturated fatty acid
ISSN号	ISSN: 1432-0614
中文摘要	Biodiesel is an interesting alternative energy source and is used as substitute for petroleum-based diesel. Microorganisms have been used for biodiesel production due to their significant environmental and economic benefits. However, few researches have investigated the regulation of fatty acid composition of these microbial diesels. Fatty acid biosynthesis in Escherichia coli has provided a paradigm for other bacteria and plants. By overexpressing two genes (fabA and fabB) associated with unsaturated fatty acid (UFA) synthesis in E. coli, we have engineered an efficient producer of UFAs. Saturated fatty acid (SFA) contents decreased from 50.2% (the control strain) to 34.6% (the recombinant strain overexpressing fabA and fabB simultaneously) and the ratio of cis-vaccenate (18:1 Delta 11), a major UFA in E. coli, reached 51.1% in this recombinant strain. When an Arabidopsis thaliana thioesterase (AtFatA) was coexpressed with these two genes, 0.19 mmol l(-1) fatty acids was produced by this E. coli strain after 18-h culture under shake-flask conditions. Free fatty acids made up about 37.5% of total fatty acid concentration in this final engineered strain carrying fabA, fabB, and AtFatA, and the ratio of UFA/SFA reached 2.3:1. This approach offers a means to improve the fatty acid composition of microdiesel and might pave the way for production of biodiesel equivalents using engineered microorganisms in the near future.
英文摘要	Biodiesel is an interesting alternative energy source and is used as substitute for petroleum-based diesel. Microorganisms have been used for biodiesel production due to their significant environmental and economic benefits. However, few researches have investigated the regulation of fatty acid composition of these microbial diesels. Fatty acid biosynthesis in Escherichia coli has provided a paradigm for other bacteria and plants. By overexpressing two genes (fabA and fabB) associated with unsaturated fatty acid (UFA) synthesis in E. coli, we have engineered an efficient producer of UFAs. Saturated fatty acid (SFA) contents decreased from 50.2% (the control strain) to 34.6% (the recombinant strain overexpressing fabA and fabB simultaneously) and the ratio of cis-vaccenate (18:1 Delta 11), a major UFA in E. coli, reached 51.1% in this recombinant strain. When an Arabidopsis thaliana thioesterase (AtFatA) was coexpressed with these two genes, 0.19 mmol l(-1) fatty acids was produced by this E. coli strain after 18-h culture under shake-flask conditions. Free fatty acids made up about 37.5% of total fatty acid concentration in this final engineered strain carrying fabA, fabB, and AtFatA, and the ratio of UFA/SFA reached 2.3:1. This approach offers a means to improve the fatty acid composition of microdiesel and might pave the way for production of biodiesel equivalents using engineered microorganisms in the near future.
学科主题	生物基化学品
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	ACYL-ACP THIOESTERASES ; WHOLE-CELL BIOCATALYST ; BIODIESEL PRODUCTION ; ENZYMATIC SYNTHESIS ; FUEL PRODUCTION ; LIPIDS ; OVERPRODUCTION ; DESATURASE ; MICROORGANISMS ; BIOSYNTHESIS
收录类别	SCI
语种	英语
WOS记录号	WOS:000277784100028
公开日期	2011-09-09
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn//handle/337004/456]
专题	青岛生物能源与过程研究所_材料生物技术研究中心
作者单位	Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Qingdao 266101, Peoples R China
推荐引用方式 GB/T 7714	Cao, Yujin,Yang, Jianming,Xian, Mo,et al. Increasing unsaturated fatty acid contents in Escherichia coli by coexpression of three different genes[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2010,87(1):271-280.
APA	Cao, Yujin,Yang, Jianming,Xian, Mo,Xu, Xin,&Liu, Wei.(2010).Increasing unsaturated fatty acid contents in Escherichia coli by coexpression of three different genes.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,87(1),271-280.
MLA	Cao, Yujin,et al."Increasing unsaturated fatty acid contents in Escherichia coli by coexpression of three different genes".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 87.1(2010):271-280.