Rational design of true monomeric and bright photoactivatable   fluorescent proteins

doi:10.1038/NMETH.2021

CORC > 北京大学 > 化学与分子工程学院

	Rational design of true monomeric and bright photoactivatable fluorescent proteins
	Zhang, Mingshu ; Chang, Hao ; Zhang, Yongdeng ; Yu, Junwei ; Wu, Lijie ; Ji, Wei ; Chen, Juanjuan ; Liu, Bei ; Lu, Jingze ; Liu, Yingfang ; Zhang, Junlong ; Xu, Pingyong ; Xu, Tao
刊名	自然方法
	2012
关键词	LOCALIZATION MICROSCOPY MARKER PROTEIN LIVE CELLS SUPERRESOLUTION EOSFP CHANNEL ORANGE PROBES ORAI1 STIM1
DOI	10.1038/NMETH.2021
英文摘要	Monomeric (m) Eos2 is an engineered photoactivatable fluorescent protein widely used for super-resolution microscopy. We show that mEos2 forms oligomers at high concentrations and forms aggregates when labeling membrane proteins, limiting its application as a fusion partner. We solved the crystal structure of tetrameric mEos2 and rationally designed improved versions, mEos3.1 and mEos3.2, that are truly monomeric, are brighter, mature faster and exhibit higher photon budget and label density.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000305942200030&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemical Research Methods; SCI(E); 97; ARTICLE; 7; 727-U297; 9
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/393184]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Zhang, Mingshu,Chang, Hao,Zhang, Yongdeng,et al. Rational design of true monomeric and bright photoactivatable fluorescent proteins[J]. 自然方法,2012.
APA	Zhang, Mingshu.,Chang, Hao.,Zhang, Yongdeng.,Yu, Junwei.,Wu, Lijie.,...&Xu, Tao.(2012).Rational design of true monomeric and bright photoactivatable fluorescent proteins.自然方法.
MLA	Zhang, Mingshu,et al."Rational design of true monomeric and bright photoactivatable fluorescent proteins".自然方法 (2012).