Molecular cloning and characterization of GbDXS and GbGGPPS gene   promoters from Ginkgo biloba

doi:10.4238/2013.February.4.3

CORC > 北京大学 > 化学与分子工程学院

	Molecular cloning and characterization of GbDXS and GbGGPPS gene promoters from Ginkgo biloba
	Xu, F. ; Huang, X. H. ; Li, L. L. ; Deng, G. ; Cheng, H. ; Rong, X. F. ; Li, J. B. ; Cheng, S. Y.
刊名	genetics and molecular research
	2013
关键词	Molecular cloning GbDXS GbGGPPS Promoter Cis-acting elements Ginkgo biloba TRANSCRIPTION FACTOR ARABIDOPSIS EXPRESSION SYNTHASE IDENTIFICATION ELEMENTS PATHWAY RICE BOX
DOI	10.4238/2013.February.4.3
英文摘要	Ginkgolides are key pharmaceutical components in Ginkgo biloba leaves. 1-Deoxy-D-xylulose-5-phosphate synthase (GbDXS) and geranylgeranyl pyrophosphate (GbGGPPS) genes are critical genes involved in ginkgolide biosynthesis. In this study, the promoters of GbDXS and GGPPS, with 676 and 570 bp in length, respectively, were cloned by chromosome walking. The cis-elements of GbDXS and GbGGPPS promoters were predicted and analyzed by the plant cis-acting regulatory element (CARE) database. We found some major cis-elements in the sequence of GbDXS and GbGGPPS promoters. The GbDXS promoter has 3 TATA boxes, 10 CAAT boxes, 6 GATA boxes, and 1 I box. The GbGGPPS promoter has 1 TATA box, 6 CAAT boxes, 6 GATA boxes, and 4 I boxes. Furthermore, some stress-related cis-elements in the promoters of GbDXS and GbGGPPS were found to be light-regulated elements, including sequences over-represented in light-induced promoters (SORLIP1-AT), GATA box, and I box, a gibberellin-responsive element (WRKY), salicylic acid-induced (GT-1), cold-and dehydration-responsive (MYC-Core), and copper-inducible (CURE-Core). Further analyses of these cis-elements will aid in elucidating the molecular mechanisms regulating the expression of the GbDXS and GbGGPPS genes during ginkgolide accumulation in G. biloba.; Biochemistry & Molecular Biology; Genetics & Heredity; SCI(E); 0; ARTICLE; 1; 293-301; 12
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/392296]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Xu, F.,Huang, X. H.,Li, L. L.,et al. Molecular cloning and characterization of GbDXS and GbGGPPS gene promoters from Ginkgo biloba[J]. genetics and molecular research,2013.
APA	Xu, F..,Huang, X. H..,Li, L. L..,Deng, G..,Cheng, H..,...&Cheng, S. Y..(2013).Molecular cloning and characterization of GbDXS and GbGGPPS gene promoters from Ginkgo biloba.genetics and molecular research.
MLA	Xu, F.,et al."Molecular cloning and characterization of GbDXS and GbGGPPS gene promoters from Ginkgo biloba".genetics and molecular research (2013).