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	High-level Production of Bovine Enterokinase LightChain Using Fed-batches by Recombinant Pichia pastoris
	J.-G. Zhang ; X.-D. Wang ; X.-Z. Mao ; D.-Z. Wei
刊名	Chemical and Biochemical Engineering Quarterly
	2009
卷号	23期号:2页码:219–224
关键词	High cell mass concentration fermentation Pichia pastoris enterokinase amount of methanol consumption
中文摘要	A recombinant Pichia pastoris containing enterokinase light chain gene from bovine was cultured in flasks firstly at different pH, methanol addition, and cell mass concentration for enterokinase production. Activity of enterokinase increased as cell mass concentration increased at more than phi = 6% methanol added. No significant change of activity of enterokinase was observed when methanol added was less than phi = 4%. No activity of enterokinase lost after 120 h conservation at different pH. Secondly, high-level enterokinase production was achieved in 3.7 L bioreactor at pH 4.0, phi = 0.6% methanol, p = 52.5 kPa. Activity of enterokinase was not cell mass concentration dependent at phi = 0.6% methanol in bioreactor. Yield of enterokinase was 479.99 mg L(-1) after 97.5 h induction. Maintenance coefficient and methanol consumed were calculated to analyse the enterokinase production.
学科主题	生物反应工程
语种	英语
公开日期	2011-09-09
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn//handle/337004/416]
专题	青岛生物能源与过程研究所_生物反应工程团队
推荐引用方式 GB/T 7714	J.-G. Zhang,X.-D. Wang,X.-Z. Mao,et al. High-level Production of Bovine Enterokinase LightChain Using Fed-batches by Recombinant Pichia pastoris[J]. Chemical and Biochemical Engineering Quarterly,2009,23(2):219–224.
APA	J.-G. Zhang,X.-D. Wang,X.-Z. Mao,&D.-Z. Wei.(2009).High-level Production of Bovine Enterokinase LightChain Using Fed-batches by Recombinant Pichia pastoris.Chemical and Biochemical Engineering Quarterly,23(2),219–224.
MLA	J.-G. Zhang,et al."High-level Production of Bovine Enterokinase LightChain Using Fed-batches by Recombinant Pichia pastoris".Chemical and Biochemical Engineering Quarterly 23.2(2009):219–224.

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