N-6-methyladenosine-dependent regulation of messenger RNA stability

doi:10.1038/nature12730

CORC > 北京大学 > 化学与分子工程学院

	N-6-methyladenosine-dependent regulation of messenger RNA stability
	Wang, Xiao ; Lu, Zhike ; Gomez, Adrian ; Hon, Gary C. ; Yue, Yanan ; Han, Dali ; Fu, Ye ; Parisien, Marc ; Dai, Qing ; Jia, Guifang ; Ren, Bing ; Pan, Tao ; He, Chuan
刊名	nature
	2014
关键词	PROCESSING BODIES PROTEIN-SYNTHESIS BINDING PROTEIN PAR-CLIP SITES DOMAIN GENE N6-METHYLADENOSINE TRANSLATION METHYLATION
DOI	10.1038/nature12730
英文摘要	N-6-methyladenosine (m(6)A) is the most prevalent internal (non-cap) modification present in the messenger RNA of all higher eukaryotes(1,2). Although essential to cell viability and development(3-5), the exact role of m(6)A modification remains to be determined. The recent discovery of two m(6)A demethylases in mammalian cells highlighted the importance of m(6)A in basic biological functions and disease(6-8). Here we show that m(6)A is selectively recognized by the human YTH domain family 2 (YTHDF2) 'reader' protein to regulate mRNA degradation. We identified over 3,000 cellular RNA targets of YTHDF2, most of which are mRNAs, but which also include non-coding RNAs, with a conserved core motif of G(m(6)A)C. We further establish the role of YTHDF2 in RNA metabolism, showing that binding of YTHDF2 results in the localization of bound mRNA from the translatable pool to mRNA decay sites, such as processing bodies(9). The carboxy-terminal domain of YTHDF2 selectively binds to m(6)A-containing mRNA, whereas the amino-terminal domain is responsible for the localization of the YTHDF2-mRNA complex to cellular RNA decay sites. Our results indicate that the dynamic m(6)A modification is recognized by selectively binding proteins to affect the translation status and lifetime of mRNA.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000329163300035&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Multidisciplinary Sciences; SCI(E); 123; ARTICLE; chuanhe@uchicago.edu; 7481; 117-+; 505
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/390185]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Wang, Xiao,Lu, Zhike,Gomez, Adrian,et al. N-6-methyladenosine-dependent regulation of messenger RNA stability[J]. nature,2014.
APA	Wang, Xiao.,Lu, Zhike.,Gomez, Adrian.,Hon, Gary C..,Yue, Yanan.,...&He, Chuan.(2014).N-6-methyladenosine-dependent regulation of messenger RNA stability.nature.
MLA	Wang, Xiao,et al."N-6-methyladenosine-dependent regulation of messenger RNA stability".nature (2014).