Palladium-triggered deprotection chemistry for protein activation in   living cells

doi:10.1038/NCHEM.1887

CORC > 北京大学 > 化学与分子工程学院

	Palladium-triggered deprotection chemistry for protein activation in living cells
	Li, Jie ; Yu, Juntao ; Zhao, Jingyi ; Wang, Jie ; Zheng, Siqi ; Lin, Shixian ; Chen, Long ; Yang, Maiyun ; Jia, Shang ; Zhang, Xiaoyu ; Chen, Peng R.
刊名	nature chemistry
	2014
关键词	GENETIC-CODE MAMMALIAN-CELLS PHOSPHOTHREONINE LYASE SMALL MOLECULES LYSINE SITE PHOSPHORYLATION CATALYSIS PATHWAYS TYROSINE
DOI	10.1038/NCHEM.1887
英文摘要	Employing small molecules or chemical reagents to modulate the function of an intracellular protein, particularly in a gain-of-function fashion, remains a challenge. In contrast to inhibitor-based loss-of-function approaches, methods based on a gain of function enable specific signalling pathways to be activated inside a cell. Here we report a chemical rescue strategy that uses a palladium-mediated deprotection reaction to activate a protein within living cells. We identify biocompatible and efficient palladium catalysts that cleave the propargyl carbamate group of a protected lysine analogue to generate a free lysine. The lysine analogue can be genetically and site-specifically incorporated into a protein, which enables control over the reaction site. This deprotection strategy is shown to work with a range of different cell lines and proteins. We further applied this biocompatible protection group/catalyst pair for caging and subsequent release of a crucial lysine residue in a bacterial Type III effector protein within host cells, which reveals details of its virulence mechanism.; Chemistry, Multidisciplinary; SCI(E); 24; ARTICLE; pengchen@pku.edu.cn; 4; 352-361; 6
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/389854]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Li, Jie,Yu, Juntao,Zhao, Jingyi,et al. Palladium-triggered deprotection chemistry for protein activation in living cells[J]. nature chemistry,2014.
APA	Li, Jie.,Yu, Juntao.,Zhao, Jingyi.,Wang, Jie.,Zheng, Siqi.,...&Chen, Peng R..(2014).Palladium-triggered deprotection chemistry for protein activation in living cells.nature chemistry.
MLA	Li, Jie,et al."Palladium-triggered deprotection chemistry for protein activation in living cells".nature chemistry (2014).