Small molecules blocking the entry of severe acute respiratory syndrome   coronavirus into host cells

doi:10.1128/JVI.78.20.11334-11339.2004

CORC > 北京大学 > 化学与分子工程学院

	Small molecules blocking the entry of severe acute respiratory syndrome coronavirus into host cells
	Yi, L ; Li, ZQ ; Yuan, KH ; Qu, XX ; Chen, J ; Wang, GW ; Zhang, H ; Luo, HP ; Zhu, LL ; Jiang, PF ; Chen, LR ; Shen, Y ; Luo, M ; Zuo, GY ; Hu, JH ; Duan, DL ; Nie, YC ; Shi, XL ; Wang, W ; Han, Y ; Li, TS ; Liu, YQ ; Ding, MX ; Deng, HK ; Xu, XJ
刊名	病毒学杂志
	2004
关键词	FRONTAL AFFINITY-CHROMATOGRAPHY HEPATITIS-C-VIRUS SARS-ASSOCIATED CORONAVIRUS CHINESE MEDICINAL HERBS SYNCYTIAL VIRUS MASS-SPECTROMETRY HIV-1 INFECTION SPIKE GLYCOPROTEIN INHIBITS ENTRY IN-VITRO
DOI	10.1128/JVI.78.20.11334-11339.2004
英文摘要	Severe acute respiratory syndrome coronavirus (SARS-CoV) is the pathogen of SARS, which caused a global panic in 2003. We describe here the screening of Chinese herbal medicine-based, novel small molecules that bind avidly with the surface spike protein of SARS-CoV and thus can interfere with the entry of the virus to its host cells. We achieved this by using a two-step screening method consisting of frontal affinity chromatography-mass spectrometry coupled with a viral infection assay based on a human immunodeficiency virus (HIV)-luc/SARS pseudotyped virus. Two small molecules, tetra-O-galloyl-beta-D-glucose (TGG) and luteolin, were identified, whose anti-SARS-CoV activities were confirmed by using a wild-type SARS-CoV infection system. TGG exhibits prominent anti-SARS-CoV activity with a 50% effective concentration of 4.5 muM and a selective index of 240.0. The two-step screening method described here yielded several small molecules that can be used for developing new classes of anti-SARS-CoV drugs and is potentially useful for the high-throughput screening of drugs inhibiting the entry of HIV, hepatitis C virus, and other insidious viruses into their host cells.; Virology; SCI(E); PubMed; 39; ARTICLE; 20; 11334-11339; 78
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/346545]
专题	化学与分子工程学院生命科学学院
推荐引用方式 GB/T 7714	Yi, L,Li, ZQ,Yuan, KH,et al. Small molecules blocking the entry of severe acute respiratory syndrome coronavirus into host cells[J]. 病毒学杂志,2004.
APA	Yi, L.,Li, ZQ.,Yuan, KH.,Qu, XX.,Chen, J.,...&Xu, XJ.(2004).Small molecules blocking the entry of severe acute respiratory syndrome coronavirus into host cells.病毒学杂志.
MLA	Yi, L,et al."Small molecules blocking the entry of severe acute respiratory syndrome coronavirus into host cells".病毒学杂志 (2004).