A protein engineered to bind uranyl selectively and with femtomolar   affinity

doi:10.1038/NCHEM.1856

CORC > 北京大学 > 化学与分子工程学院

	A protein engineered to bind uranyl selectively and with femtomolar affinity
	Zhou, Lu ; Bosscher, Mike ; Zhang, Changsheng ; Oezcubukcu, Salih ; Zhang, Liang ; Zhang, Wen ; Li, Charles J. ; Liu, Jianzhao ; Jensen, Mark P. ; Lai, Luhua ; He, Chuan
刊名	nature chemistry
	2014
关键词	COORDINATION CHEMISTRY ENZYME DESIGN SEA-WATER URANIUM EXTRACTION SEAWATER ION METALLOPROTEINS FORMS
DOI	10.1038/NCHEM.1856
英文摘要	Uranyl (UO22+), the predominant aerobic form of uranium, is present in the ocean at a concentration of similar to 3.2 parts per 10(9) (13.7 nM); however, the successful enrichment of uranyl from this vast resource has been limited by the high concentrations of metal ions of similar size and charge, which makes it difficult to design a binding motif that is selective for uranyl. Here we report the design and rational development of a uranyl-binding protein using a computational screening process in the initial search for potential uranyl-binding sites. The engineered protein is thermally stable and offers very high affinity and selectivity for uranyl with a K-d of 7.4 femtomolar (fM) and >10,000-fold selectivity over other metal ions. We also demonstrated that the uranyl-binding protein can repeatedly sequester 30-60% of the uranyl in synthetic sea water. The chemical strategy employed here may be applied to engineer other selective metal-binding proteins for biotechnology and remediation applications.; Chemistry, Multidisciplinary; SCI(E); PubMed; 13; ARTICLE; lhlai@pku.edu.cn; chuanhe@uchicago.edu; 3; 236-241; 6
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/342374]
专题	化学与分子工程学院生命科学学院
推荐引用方式 GB/T 7714	Zhou, Lu,Bosscher, Mike,Zhang, Changsheng,et al. A protein engineered to bind uranyl selectively and with femtomolar affinity[J]. nature chemistry,2014.
APA	Zhou, Lu.,Bosscher, Mike.,Zhang, Changsheng.,Oezcubukcu, Salih.,Zhang, Liang.,...&He, Chuan.(2014).A protein engineered to bind uranyl selectively and with femtomolar affinity.nature chemistry.
MLA	Zhou, Lu,et al."A protein engineered to bind uranyl selectively and with femtomolar affinity".nature chemistry (2014).