Dynamic sialylation in transforming growth factor-�� (TGF-��)-induced epithelial to mesenchymal transition

doi:10.1074/jbc.M115.636969

CORC > 北京大学 > 化学与分子工程学院

	Dynamic sialylation in transforming growth factor-�� (TGF-��)-induced epithelial to mesenchymal transition
	Du, Jun ; Hong, Senlian ; Dong, Lu ; Cheng, Bo ; Lin, Liang ; Zhao, Bing ; Chen, Ye-Guang ; Chen, Xing
刊名	journal of biological chemistry
	2015
DOI	10.1074/jbc.M115.636969
英文摘要	Epithelial-mesenchymal transition (EMT) is a fundamental process in embryonic development and organ formation. Aberrant regulation of EMT often leads to tumor progression. Changes in cell surface sialylation have recently been implicated in mediating EMT. Herein we report the visualization of dynamic changes of sialylation and glycoproteomic analysis of newly synthesized sialylated proteins in EMT by metabolic labeling of sialylated glycans with azides, followed by click labeling with fluorophores or affinity tags. We discovered that sialylation was down-regulated during EMT but then reverted and up-regulated in the mesenchymal state after EMT, accompanied by mRNA expression level changes of genes involved in the sialic acid biosynthesis. Quantitative proteomic analysis identified a list of sialylated proteins whose biosynthesis was dynamically regulated during EMT. Sialylation of cell surface adherent receptor integrin ??4 was found to be down-regulated, which may regulate integrin functions during EMT. Furthermore, a global sialylation inhibitor was used to probe the functional role of sialylation during EMT. We found that inhibition of sialylation promoted EMT. Taken together, our findings suggest the important role of sialylation in regulating EMT and imply its possible function in related pathophysiological events, such as cancer metastasis. ? 2015 by The American Society for Biochemistry and Molecular Biology, Inc.; SCI(E); EI; 0; ARTICLE; ygchen@tsinghua.edu.cn; xingchen@pku.edu.cn; 19; 12000-12013; 290
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/328212]
专题	化学与分子工程学院生命科学学院
推荐引用方式 GB/T 7714	Du, Jun,Hong, Senlian,Dong, Lu,et al. Dynamic sialylation in transforming growth factor-�� (TGF-��)-induced epithelial to mesenchymal transition[J]. journal of biological chemistry,2015.
APA	Du, Jun.,Hong, Senlian.,Dong, Lu.,Cheng, Bo.,Lin, Liang.,...&Chen, Xing.(2015).Dynamic sialylation in transforming growth factor-�� (TGF-��)-induced epithelial to mesenchymal transition.journal of biological chemistry.
MLA	Du, Jun,et al."Dynamic sialylation in transforming growth factor-�� (TGF-��)-induced epithelial to mesenchymal transition".journal of biological chemistry (2015).