A high-throughput imaging system to quantitatively analyze the growth   dynamics of plant seedlings

doi:10.1039/c2ib20020a

CORC > 北京大学 > 化学与分子工程学院

	A high-throughput imaging system to quantitatively analyze the growth dynamics of plant seedlings
	Men, Yongfan ; Yu, Qiang ; Chen, Zitian ; Wang, Jianbin ; Huang, Yanyi ; Guo, Hongwei
刊名	integrative biology
	2012
关键词	TEMPORAL COLONIZATION PATTERNS APICAL HOOK DEVELOPMENT ARABIDOPSIS-THALIANA HYPOCOTYL GROWTH KINETIC-ANALYSIS GENE-EXPRESSION BLUE-LIGHT ETHYLENE RESPONSES AUXIN
DOI	10.1039/c2ib20020a
英文摘要	Most current methods for analyzing the growth rate of plant seedlings are limited to low-throughput experimental configurations. We have developed an automatic system to investigate the dynamics of the growth of hypocotyls using Arabidopsis as model. This system is able to capture time-lapse infrared images of 24 seedlings automatically, with a spatial resolution of 2 mm per pixel and temporal interval of 5 min. Seedling length is rapidly calculated using automated geometric image-processing algorithms. With this high-throughput platform, we have investigated the genotype dependent difference of growth patterns, as well as the response to plant hormone - ethylene. Our analyses suggest that cytoskeleton function is not required in ethylene-induced hypocotyl inhibition. This novel integrative method can be applied to large-scale dynamic screening of plants, as well as any other image-based biological studies related to dynamic growth.; Cell Biology; SCI(E); PubMed; 3; ARTICLE; 8; 945-952; 4
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/315682]
专题	化学与分子工程学院生命科学学院工学院
推荐引用方式 GB/T 7714	Men, Yongfan,Yu, Qiang,Chen, Zitian,et al. A high-throughput imaging system to quantitatively analyze the growth dynamics of plant seedlings[J]. integrative biology,2012.
APA	Men, Yongfan,Yu, Qiang,Chen, Zitian,Wang, Jianbin,Huang, Yanyi,&Guo, Hongwei.(2012).A high-throughput imaging system to quantitatively analyze the growth dynamics of plant seedlings.integrative biology.
MLA	Men, Yongfan,et al."A high-throughput imaging system to quantitatively analyze the growth dynamics of plant seedlings".integrative biology (2012).