一种新型结构的金属硫蛋白Pb-MT

doi:10.3321/j.issn:0251-0790.1999.02.032

CORC > 北京大学 > 化学与分子工程学院

	一种新型结构的金属硫蛋白Pb-MT; A Novel Structure Motif of MetallothioneinPb-MT'
	贺卫国 ; 褚德萤 ; 杨静岳 ; 姚丹凤 ; 邵美成
刊名	高等学校化学学报
	1999
关键词	金属硫蛋白铅离子紫外-可见光谱圆二色谱新型结构 metallothionein lead (II) ion UV-Vis spectrum circular dichroism novel structure
DOI	10.3321/j.issn:0251-0790.1999.02.032
英文摘要	金属硫蛋白(metallothioneins,简称MTs)是一大类富含巯基的小分子蛋白质(分子量<9000),具有很强的金属结合能力,哺乳动物MT每分子可结合7个二价金属离子或12个一价金属离子.MT的结构一直是科学家关注的热点,其中哺乳动物Cd7-MT和Cd5-,Zn2-MT的三维结构已分别用NMR[1]和X射线衍射法[2]测定.研究结果表明,二价金属离子在MT中全部与硫原子四面体配位,并通过桥联的硫原子形成M2+4S11和M2+3S9两个金属-硫簇,相应组成α-、β-两个结构域.; Lead(II)-binding behaviors of rabbit liver thionein2 (apo-MT2) has been studied by monitoring the changes of their electronic absorption(UV) and circular dichroism(CD) spectra, Two MT species, Pb-7-MT and Pb-7-MT', are observed upon the binding of Pb to ape-MT at different pH. The formation of Pb-7-MT or Pb-7-MT' is closely related to the amount of deprotoned sulfhydryl groups. Pb-7-MT is formed only at high pH characterized by UV 340 nm shoulder and CD 245 nm band. Then the formation of a new lead-metallothionein complex. namely Pb MT', is observed at low pH (pH < 6). This new species is characterized by a strong circular dichroism(CD) cotton effect at 395 nm(-), 310 nm(-) peaks and 350 nm (+), 250 nm (+) double-hump and a unique electronic absorption(UV) spectrum at 325 nm, 375 nm and 400 nm shoulder. From their characteristic peaks in UV and CD spectra, it is deduced that Pb2+ in Pb-7-MT is coordinated by four sulfhydryl groups in Td microsymmetry, while multiple Pb2+ coordination including two-coordination maybe present in Pb-7-MT', This is a novel structure motif that was not found previously for MT species.; 中国科学院资助项目; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000079044500020&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; SCI(E); 中文核心期刊要目总览(PKU); 中国科学引文数据库(CSCD); 3; 2; 248; 20
语种	中文
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/312801]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	贺卫国,褚德萤,杨静岳,等. 一种新型结构的金属硫蛋白Pb-MT, A Novel Structure Motif of MetallothioneinPb-MT'[J]. 高等学校化学学报,1999.
APA	贺卫国,褚德萤,杨静岳,姚丹凤,&邵美成.(1999).一种新型结构的金属硫蛋白Pb-MT.高等学校化学学报.
MLA	贺卫国,et al."一种新型结构的金属硫蛋白Pb-MT".高等学校化学学报 (1999).