Characterizing DNA Condensation and Conformational Changes in Organic   Solvents

doi:10.1371/journal.pone.0013308

CORC > 北京大学 > 化学与分子工程学院

	Characterizing DNA Condensation and Conformational Changes in Organic Solvents
	Ke, Fuyou ; Luu, Yen Kim ; Hadjiargyrou, Michael ; Liang, Dehai
刊名	plos one
	2010
关键词	DIBLOCK COPOLYMER VESICLES ATOMIC-FORCE MICROSCOPY CALF THYMUS DNA LIGHT-SCATTERING GENE DELIVERY PLASMID DNA IONIC-STRENGTH ETHANOL ENCAPSULATION COMPLEXES
DOI	10.1371/journal.pone.0013308
英文摘要	Organic solvents offer a new approach to formulate DNA into novel structures suitable for gene delivery. In this study, we examined the in situ behavior of DNA in N, N-dimethylformamide (DMF) at low concentration via laser light scattering (LLS), TEM, UV absorbance and Zeta potential analysis. Results revealed that, in DMF, a 21bp oligonucleotide remained intact, while calf thymus DNA and supercoiled plasmid DNA were condensed and denatured. During condensation and denaturation, the size was decreased by a factor of 8-10, with calf thymus DNA forming spherical globules while plasmid DNA exhibited a toroid-like conformation. In the condensed state, DNA molecules were still able to release the counterions to be negatively charged, indicating that the condensation was mainly driven by the excluded volume interactions. The condensation induced by DMF was reversible for plasmid DNA but not for calf thymus DNA. When plasmid DNA was removed from DMF and resuspended in an aqueous solution, the DNA was quickly regained a double stranded configuration. These findings provide further insight into the behavior and condensation mechanism of DNA in an organic solvent and may aid in developing more efficient non-viral gene delivery systems.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000282748100030&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Multidisciplinary Sciences; SCI(E); PubMed; 12; ARTICLE; 10; e13308; 5
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/312599]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Ke, Fuyou,Luu, Yen Kim,Hadjiargyrou, Michael,et al. Characterizing DNA Condensation and Conformational Changes in Organic Solvents[J]. plos one,2010.
APA	Ke, Fuyou,Luu, Yen Kim,Hadjiargyrou, Michael,&Liang, Dehai.(2010).Characterizing DNA Condensation and Conformational Changes in Organic Solvents.plos one.
MLA	Ke, Fuyou,et al."Characterizing DNA Condensation and Conformational Changes in Organic Solvents".plos one (2010).