Mechanism-Based Design of a Photoactivatable Firefly Luciferase

doi:10.1021/ja4013535

CORC > 北京大学 > 化学与分子工程学院

	Mechanism-Based Design of a Photoactivatable Firefly Luciferase
	Zhao, Jingyi ; Lin, Shixian ; Huang, Yong ; Zhao, Jing ; Chen, Peng R.
刊名	journal of the american chemical society
	2013
关键词	MAMMALIAN-CELLS PHOTOCAGED TYROSINE GENETIC-CODE ATP LEVELS PROTEIN FLUORESCENCE PHOTOCONTROL LYSINE ASSAY
DOI	10.1021/ja4013535
英文摘要	We developed a photoactivatable firefly luciferase (pfLuc) whose activation can be controlled by light. A photocaged Lys analogue was site-specifically incorporated into fLuc to replace its key catalytic Lys residue, Lys529, rendering fLuc inactive until light-triggered removal of the caging group. This photoinduced gain of luminescence provides a facile approach for assessing the photolysis efficiency of this valuable photo-sensitive Lys analogue within the context of its carrier protein in vitro and in living cells. We further took advantage of the spatial and temporal activation feature of pfLuc for intracellular measurement of labile ATP levels without impairment of cellular physiology.; Chemistry, Multidisciplinary; SCI(E); EI; PubMed; 11; ARTICLE; 20; 7410-7413; 135
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/312558]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Zhao, Jingyi,Lin, Shixian,Huang, Yong,et al. Mechanism-Based Design of a Photoactivatable Firefly Luciferase[J]. journal of the american chemical society,2013.
APA	Zhao, Jingyi,Lin, Shixian,Huang, Yong,Zhao, Jing,&Chen, Peng R..(2013).Mechanism-Based Design of a Photoactivatable Firefly Luciferase.journal of the american chemical society.
MLA	Zhao, Jingyi,et al."Mechanism-Based Design of a Photoactivatable Firefly Luciferase".journal of the american chemical society (2013).