Both piston-like and rotational motions are present in bacterial   chemoreceptor signaling

doi:10.1038/srep08640

CORC > 北京大学 > 化学与分子工程学院

	Both piston-like and rotational motions are present in bacterial chemoreceptor signaling
	Yu, Daqi ; Ma, Xiaomin ; Tu, Yuhai ; Lai, Luhua
刊名	scientific reports
	2015
关键词	LIGAND-BINDING DOMAIN COLI SERINE CHEMORECEPTOR ASPARTATE RECEPTOR ESCHERICHIA-COLI CONFORMATIONAL-CHANGES 3-DIMENSIONAL STRUCTURE SALMONELLA-TYPHIMURIUM CHEMOTAXIS RECEPTORS CROSS-LINKING PROTEINS
DOI	10.1038/srep08640
英文摘要	Bacterial chemotaxis signaling is triggered by binding of chemo-effectors to the membrane-bound chemoreceptor dimers. Though much is known about the structure of the chemoreceptors, details of the receptor dynamics and their effects on signaling are still unclear. Here, by using molecular dynamics simulations and principle component analysis, we study the dynamics of the periplasmic domain of aspartate chemoreceptor Tar dimer and its conformational changes when binding to different ligands (attractant, antagonist, and two attractant molecules). We found two dominant components (modes) in the receptor dynamics: a relative rotation of the two Tar monomers and a piston-like up-and-down sliding movement of the alpha 4 helix. These two modes are highly correlated. Binding of one attractant molecule to the Tar dimer induced both significant piston-like downward movements of the a4 helix and strong relative rotations of the two Tar monomers, while binding of an antagonist or the symmetric binding of two attractant molecules to a Tar dimer suppresses both modes. The anti-symmetric effects of the relative rotation mode also explained the negative cooperativity between the two binding pockets. Our results suggest a mechanism of coupled rotation and piston-like motion for bacterial chemoreceptor signaling.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000350298300007&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Multidisciplinary Sciences; SCI(E); PubMed; 8; ARTICLE; yuhai@us.ibm.com; lhlai@pku.edu.cn; 8640; 5
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/312541]
专题	化学与分子工程学院生命科学学院
推荐引用方式 GB/T 7714	Yu, Daqi,Ma, Xiaomin,Tu, Yuhai,et al. Both piston-like and rotational motions are present in bacterial chemoreceptor signaling[J]. scientific reports,2015.
APA	Yu, Daqi,Ma, Xiaomin,Tu, Yuhai,&Lai, Luhua.(2015).Both piston-like and rotational motions are present in bacterial chemoreceptor signaling.scientific reports.
MLA	Yu, Daqi,et al."Both piston-like and rotational motions are present in bacterial chemoreceptor signaling".scientific reports (2015).