X-ray studies on cross-linked lysozyme crystals in acetonitrile-water mixture | |
Wang, ZM ; Zhu, GY ; Huang, QC ; Qian, MX ; Shao, MC ; Jia, YS ; Tang, YQ | |
刊名 | biochimica et biophysica acta protein structure and molecular enzymology
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1998 | |
关键词 | lysozyme organic medium X-ray crystallography EGG-WHITE LYSOZYME ENZYMATIC CATALYSIS ORGANIC-SOLVENTS REFINEMENT ENVIRONMENTS FLEXIBILITY DYNAMICS PROTEINS BINDING MEDIA |
DOI | 10.1016/S0167-4838(98)00027-2 |
英文摘要 | Tetragonal crystals of hen egg white lysozyme were cross-linked and subjected to X-ray diffraction study in acetonitrile-water media with different acetonitrile concentrations. Crystals in neat acetonitrile did not scatter X-ray well. Structures of crystals in neat water, in 90% and 95% acetonitrile, and crystal back-soaked from acetonitrile to water, were determined to about 2 Angstrom resolution. For crystals in both 90% and 95% acetonitrile, only one protein-bond acetonitrile molecule is found in the active site cleft, and its location and binding-protein mode is similar to the C subunit of polysaccharide. The alteration in conformation and hydrogen-bond pattern involving water as solvent causes the reduction of the protein's flexibility in organic media. The back-soaked crystal regained its ordinary three-dimensional structure in water. (C) 1998 Elsevier Science B.V. All rights reserved.; Biochemistry & Molecular Biology; Biophysics; SCI(E); 23; 2; 335-344; 1384 |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://ir.pku.edu.cn/handle/20.500.11897/257848] ![]() |
专题 | 化学与分子工程学院 |
推荐引用方式 GB/T 7714 | Wang, ZM,Zhu, GY,Huang, QC,et al. X-ray studies on cross-linked lysozyme crystals in acetonitrile-water mixture[J]. biochimica et biophysica acta protein structure and molecular enzymology,1998. |
APA | Wang, ZM.,Zhu, GY.,Huang, QC.,Qian, MX.,Shao, MC.,...&Tang, YQ.(1998).X-ray studies on cross-linked lysozyme crystals in acetonitrile-water mixture.biochimica et biophysica acta protein structure and molecular enzymology. |
MLA | Wang, ZM,et al."X-ray studies on cross-linked lysozyme crystals in acetonitrile-water mixture".biochimica et biophysica acta protein structure and molecular enzymology (1998). |
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