X-ray studies on cross-linked lysozyme crystals in acetonitrile-water   mixture

doi:10.1016/S0167-4838(98)00027-2

CORC > 北京大学 > 化学与分子工程学院

	X-ray studies on cross-linked lysozyme crystals in acetonitrile-water mixture
	Wang, ZM ; Zhu, GY ; Huang, QC ; Qian, MX ; Shao, MC ; Jia, YS ; Tang, YQ
刊名	biochimica et biophysica acta protein structure and molecular enzymology
	1998
关键词	lysozyme organic medium X-ray crystallography EGG-WHITE LYSOZYME ENZYMATIC CATALYSIS ORGANIC-SOLVENTS REFINEMENT ENVIRONMENTS FLEXIBILITY DYNAMICS PROTEINS BINDING MEDIA
DOI	10.1016/S0167-4838(98)00027-2
英文摘要	Tetragonal crystals of hen egg white lysozyme were cross-linked and subjected to X-ray diffraction study in acetonitrile-water media with different acetonitrile concentrations. Crystals in neat acetonitrile did not scatter X-ray well. Structures of crystals in neat water, in 90% and 95% acetonitrile, and crystal back-soaked from acetonitrile to water, were determined to about 2 Angstrom resolution. For crystals in both 90% and 95% acetonitrile, only one protein-bond acetonitrile molecule is found in the active site cleft, and its location and binding-protein mode is similar to the C subunit of polysaccharide. The alteration in conformation and hydrogen-bond pattern involving water as solvent causes the reduction of the protein's flexibility in organic media. The back-soaked crystal regained its ordinary three-dimensional structure in water. (C) 1998 Elsevier Science B.V. All rights reserved.; Biochemistry & Molecular Biology; Biophysics; SCI(E); 23; 2; 335-344; 1384
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/257848]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Wang, ZM,Zhu, GY,Huang, QC,et al. X-ray studies on cross-linked lysozyme crystals in acetonitrile-water mixture[J]. biochimica et biophysica acta protein structure and molecular enzymology,1998.
APA	Wang, ZM.,Zhu, GY.,Huang, QC.,Qian, MX.,Shao, MC.,...&Tang, YQ.(1998).X-ray studies on cross-linked lysozyme crystals in acetonitrile-water mixture.biochimica et biophysica acta protein structure and molecular enzymology.
MLA	Wang, ZM,et al."X-ray studies on cross-linked lysozyme crystals in acetonitrile-water mixture".biochimica et biophysica acta protein structure and molecular enzymology (1998).