Perfusate oxygen and carbon dioxide concentration influence basal microdialysate levels of striatal glucose and lactate in conscious rats | |
Wang, L ; Li, YZ ; Han, HW ; Liu, GQ ; Osborne, PG | |
刊名 | 神经科学快报
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2003 | |
关键词 | perfusion hyperoxia hypoxia metabolism glucose lactate CEREBRAL BLOOD-FLOW BRAIN GLUTAMATE CORTEX DOPAMINE HYPOXIA TENSION |
DOI | 10.1016/S0304-3940(03)00448-8 |
英文摘要 | O-2 concentration ([O-2]) in air equilibrated solutions at room temperature is three fold higher than that in brain extracellular fluid (ECF), and CO2 concentration ([CO2]) is 100 times lower. Using microdialysis the ECF is routinely dialyzed against glucose free isotonic perfusates containing 200 muM O-2 and 10 muM CO2. In conscious rats, 2 days after probe implantation, decreasing perfusate [O-2] from 200 to 68 muM (physiologic level) for 60 min, while maintaining a low [CO2] (10 muM), increased striatal dialysate glucose and lactate by 12% and 33%, respectively. The same, protocol on the third day essentially had no effect on monoamine metabolites. Decreasing [O-2] concurrent with increasing [CO2] to 1.3 mM (physiologic level) for 60 min increased glucose and lactate by 17% and 37%, respectively. This study demonstrates for dialysis studies of glucose and lactate, perfusates that mimic physiologic ECF [O-2] and [CO2] are more appropriate. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.; Neurosciences; SCI(E); 2; ARTICLE; 2; 91-94; 344 |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://ir.pku.edu.cn/handle/20.500.11897/256508] ![]() |
专题 | 化学与分子工程学院 |
推荐引用方式 GB/T 7714 | Wang, L,Li, YZ,Han, HW,et al. Perfusate oxygen and carbon dioxide concentration influence basal microdialysate levels of striatal glucose and lactate in conscious rats[J]. 神经科学快报,2003. |
APA | Wang, L,Li, YZ,Han, HW,Liu, GQ,&Osborne, PG.(2003).Perfusate oxygen and carbon dioxide concentration influence basal microdialysate levels of striatal glucose and lactate in conscious rats.神经科学快报. |
MLA | Wang, L,et al."Perfusate oxygen and carbon dioxide concentration influence basal microdialysate levels of striatal glucose and lactate in conscious rats".神经科学快报 (2003). |
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