Perfusate oxygen and carbon dioxide concentration influence basal   microdialysate levels of striatal glucose and lactate in conscious rats

doi:10.1016/S0304-3940(03)00448-8

CORC > 北京大学 > 化学与分子工程学院

	Perfusate oxygen and carbon dioxide concentration influence basal microdialysate levels of striatal glucose and lactate in conscious rats
	Wang, L ; Li, YZ ; Han, HW ; Liu, GQ ; Osborne, PG
刊名	神经科学快报
	2003
关键词	perfusion hyperoxia hypoxia metabolism glucose lactate CEREBRAL BLOOD-FLOW BRAIN GLUTAMATE CORTEX DOPAMINE HYPOXIA TENSION
DOI	10.1016/S0304-3940(03)00448-8
英文摘要	O-2 concentration ([O-2]) in air equilibrated solutions at room temperature is three fold higher than that in brain extracellular fluid (ECF), and CO2 concentration ([CO2]) is 100 times lower. Using microdialysis the ECF is routinely dialyzed against glucose free isotonic perfusates containing 200 muM O-2 and 10 muM CO2. In conscious rats, 2 days after probe implantation, decreasing perfusate [O-2] from 200 to 68 muM (physiologic level) for 60 min, while maintaining a low [CO2] (10 muM), increased striatal dialysate glucose and lactate by 12% and 33%, respectively. The same, protocol on the third day essentially had no effect on monoamine metabolites. Decreasing [O-2] concurrent with increasing [CO2] to 1.3 mM (physiologic level) for 60 min increased glucose and lactate by 17% and 37%, respectively. This study demonstrates for dialysis studies of glucose and lactate, perfusates that mimic physiologic ECF [O-2] and [CO2] are more appropriate. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.; Neurosciences; SCI(E); 2; ARTICLE; 2; 91-94; 344
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/256508]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Wang, L,Li, YZ,Han, HW,et al. Perfusate oxygen and carbon dioxide concentration influence basal microdialysate levels of striatal glucose and lactate in conscious rats[J]. 神经科学快报,2003.
APA	Wang, L,Li, YZ,Han, HW,Liu, GQ,&Osborne, PG.(2003).Perfusate oxygen and carbon dioxide concentration influence basal microdialysate levels of striatal glucose and lactate in conscious rats.神经科学快报.
MLA	Wang, L,et al."Perfusate oxygen and carbon dioxide concentration influence basal microdialysate levels of striatal glucose and lactate in conscious rats".神经科学快报 (2003).