The covalent immobilization of trypsin at the galleries of layered   gamma-zirconium phosphate

doi:10.1016/S0927-7765(03)00053-5

CORC > 北京大学 > 化学与分子工程学院

	The covalent immobilization of trypsin at the galleries of layered gamma-zirconium phosphate
	Geng, LN ; Li, N ; Xiang, MH ; Wen, XF ; Xu, D ; Zhao, FL ; Li, KA
刊名	colloids and surfaces b biointerfaces
	2003
关键词	layered gamma-zirconium phosphate trypsin covalent immobilization glutaraldehyde bovine serum albumin INTERCALATION ENZYME BEADS
DOI	10.1016/S0927-7765(03)00053-5
英文摘要	Assorted trypsin immobilization trials were thoroughly conducted and compared to identify an efficient enzyme immobilization at the galleries of amine preintercalated gamma-zirconium phosphate. That was, (a) direct trypsin binding by physical adsorption, (b) coupling of enzyme to glutaraldehyde (GA) activated carrier, (c) trypsin immobilization by GA crosslinking, (d) incorporating bovine serum albumin (BSA) as a spacer during the immobilization by covalently coupling to GA activated carrier or by covalent trypsin crosslinking with GA. We reported here that covalent immobilization of trypsin by using GA as a crosslinking agent and BSA as a spacer was superior to other immobilization strategies investigated. In this study, the experimental parameters in each step of the immobilization procedure, such as the concentration of the preintercalation. agent, the crosslinking agent, the spacer and trypsin, etc., were investigated to improve the efficiency of trypsin immobilization. A reasonable enzymatic activity recovery at 75.6% and improved thermal stability were achieved. The optimum pH value and optimum temperature of immobilized enzyme were determined. (C) 2003 Elsevier Science B.V. All rights reserved.; Biophysics; Chemistry, Physical; Materials Science, Biomaterials; SCI(E); EI; 0; ARTICLE; 1-2; 99-109; 30
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/256482]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Geng, LN,Li, N,Xiang, MH,et al. The covalent immobilization of trypsin at the galleries of layered gamma-zirconium phosphate[J]. colloids and surfaces b biointerfaces,2003.
APA	Geng, LN.,Li, N.,Xiang, MH.,Wen, XF.,Xu, D.,...&Li, KA.(2003).The covalent immobilization of trypsin at the galleries of layered gamma-zirconium phosphate.colloids and surfaces b biointerfaces.
MLA	Geng, LN,et al."The covalent immobilization of trypsin at the galleries of layered gamma-zirconium phosphate".colloids and surfaces b biointerfaces (2003).