Interactions of calf thymus DNA with short chain oligoamides

CORC > 北京大学 > 化学与分子工程学院

	Interactions of calf thymus DNA with short chain oligoamides
	Du, WH ; Wang, BH ; Li, ZF ; Xiao, JH ; Yuan, G ; Huang, WQ
刊名	热化学学报
	2003
关键词	calf thymus DNA short chain oligoamide interaction binding affinity MINOR-GROOVE BASE-PAIRS BINDING RECOGNITION DISTAMYCIN COMPLEXES INTERCALATION ENTHALPY LIGANDS
英文摘要	In order to detect the biological activity of new synthetic short chain oligoamides, the present work gives the results of studies on the interaction between calf thymus DNA and short chain oligoamides: PyPyPy-gamma-Dp (ligand 1) and PyPyPyPy-gamma-Dp (ligand 2). In the circular dichroism (CD) spectra of the mixing solution of DNA with ligands a specific peak appears in the region of 300-380 nm wavelength, which indicates that both ligands I and 2 can bind calf thymus DNA specifically. H-1 NMR spectra show that aromatic ring protons of ligand I appear as an obvious down-field shift, while those protons in ligand 2 disappear since ligand 2 owns a higher binding affinity with DNA than that of ligand 1. Investigations of DNA transition by differential scanning calorimetry (DSC) reveal that DNA melting temperature rises due to binding with ligands, and that ligand 2 alters the DNA transition process more significantly. At the same time, the molar binding enthalpies of ligands with DNA are measured at 293 K. The difference between the binding affinity of ligand I and 2 with DNA implies that increasing the number of N-methylpyrrole increases both the binding site size and the binding affinity. (C) 2002 Elsevier Science B.V. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000180362300023&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Chemistry, Analytical; Chemistry, Physical; SCI(E); 4; ARTICLE; 1-2; 257-263; 395
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/256224]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Du, WH,Wang, BH,Li, ZF,et al. Interactions of calf thymus DNA with short chain oligoamides[J]. 热化学学报,2003.
APA	Du, WH,Wang, BH,Li, ZF,Xiao, JH,Yuan, G,&Huang, WQ.(2003).Interactions of calf thymus DNA with short chain oligoamides.热化学学报.
MLA	Du, WH,et al."Interactions of calf thymus DNA with short chain oligoamides".热化学学报 (2003).