Preparation of steroid antibodies and parallel detection of   multianabolic steroid abuse with conjugated hapten microarray

doi:10.1021/ac049159a

CORC > 北京大学 > 化学与分子工程学院

	Preparation of steroid antibodies and parallel detection of multianabolic steroid abuse with conjugated hapten microarray
	Du, HW ; Yuan, L ; Yang, WP ; Wu, MT ; Jun, W ; Shan, Z ; Pan, MG ; Jing, C
刊名	analytical chemistry
	2004
关键词	PROTEIN SERODIAGNOSIS
DOI	10.1021/ac049159a
英文摘要	A conjugated hapten microarray based on miniature immunoassay for fast and multiplex detection of anabolic steroids is reported for the first time. This preliminary study investigated the possibility of using a microarray technology as a multisteroid detection assay. The microarray system used eight monoclonal antibodies raised against three steroid conjugates, 4-androsten-4-chloro17beta-ol-3-one, 1,5alpha-androsten-1beta-methyl-17beta-ol-3-one, and 5beta-androsten-1-en- 17beta-ol-3-one, which were conjugated to BSA by the active ester method. In addition to 4 commercial conjugated haptens, 18 steroid-BSA conjugates were synthesized and from all these a conjugated hapten microarray was fabricated. The analyzed substances included 42 types of anabolic steroid reference materials and 28 positive urine samples. Of these, 24 anabolic steroids and 12 positive urines were successfully detected.; Chemistry, Analytical; SCI(E); EI; 20; LETTER; 20; 6166-6171; 76
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/254795]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Du, HW,Yuan, L,Yang, WP,et al. Preparation of steroid antibodies and parallel detection of multianabolic steroid abuse with conjugated hapten microarray[J]. analytical chemistry,2004.
APA	Du, HW.,Yuan, L.,Yang, WP.,Wu, MT.,Jun, W.,...&Jing, C.(2004).Preparation of steroid antibodies and parallel detection of multianabolic steroid abuse with conjugated hapten microarray.analytical chemistry.
MLA	Du, HW,et al."Preparation of steroid antibodies and parallel detection of multianabolic steroid abuse with conjugated hapten microarray".analytical chemistry (2004).