Electrochemical immunoassay of estrone at an antibody-modified   conducting polymer electrode towards immunobiosensors

doi:10.1016/j.jelechem.2006.05.002

CORC > 北京大学 > 化学与分子工程学院

	Electrochemical immunoassay of estrone at an antibody-modified conducting polymer electrode towards immunobiosensors
	Gao, Hui ; Lu, Jiayi ; Cui, Yiran ; Zhang, Xin-Xiang
刊名	journal of electroanalytical chemistry
	2006
关键词	estrone polypyrrole ferrocyanide immunoassay electrochemical detection MONOCLONAL-ANTIBODY POLYPYRROLE FILMS IMMOBILIZATION BIOSENSORS ESTRADIOL RAMAN ACID AMPLIFICATION POLAROGRAPHY
DOI	10.1016/j.jelechem.2006.05.002
英文摘要	A simple electrochemical immunoassay based on an antibody-modified conducting polymer electrode by direct electrochemical polymerization of pyrrole on a platinum electrode in an aqueous solution and simultaneous doping of the polymer with anti-estrone antibody as the dopant was demonstrated. The specific binding between estrone and anti-estrone antibody immobilized on the electrode surface was evaluated by monitoring the changes in the electrode response of potassium ferrocyanide as the redox marker and a decreased electrode response was observed upon the binding of the estrone to the polypyrrole-antibody (PPy-Ab) modified electrode surface. This change was attributed to the steric hindrance generated from the formation of immunological complex between the electrode surface and the redox marker. A direct and facile method of electrochemical detection of, estrone has been established in this way. (c) 2006 Elsevier B.V. All rights reserved.; Chemistry, Analytical; Electrochemistry; SCI(E); EI; 0; ARTICLE; 1; 88-94; 592
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/251972]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Gao, Hui,Lu, Jiayi,Cui, Yiran,et al. Electrochemical immunoassay of estrone at an antibody-modified conducting polymer electrode towards immunobiosensors[J]. journal of electroanalytical chemistry,2006.
APA	Gao, Hui,Lu, Jiayi,Cui, Yiran,&Zhang, Xin-Xiang.(2006).Electrochemical immunoassay of estrone at an antibody-modified conducting polymer electrode towards immunobiosensors.journal of electroanalytical chemistry.
MLA	Gao, Hui,et al."Electrochemical immunoassay of estrone at an antibody-modified conducting polymer electrode towards immunobiosensors".journal of electroanalytical chemistry (2006).