An electrochemical immunosensor based on agarose hydrogel films for   rapid determination of ractopamine

doi:10.1016/j.elecom.2006.10.049

CORC > 北京大学 > 化学与分子工程学院

	An electrochemical immunosensor based on agarose hydrogel films for rapid determination of ractopamine
	Shen, Li ; He, Pingli
刊名	电化学通讯
	2007
关键词	ractopamine immunosensor electrochemistry agarose hydrogel feed CHROMATOGRAPHY/TANDEM MASS-SPECTROMETRY LIQUID-CHROMATOGRAPHY AMPEROMETRIC IMMUNOSENSOR BETA-AGONIST IMMUNOASSAY URINE TISSUES SWINE HYDROCHLORIDE ZILPATEROL
DOI	10.1016/j.elecom.2006.10.049
英文摘要	A novel label-free electrochemical immunosensor for rapid determination of ractopamine was constructed by incorporating ractopamine-bovine thyroglobulin antigen in agarose hydrogel films modified on a glassy carbon electrode. Cyclic voltammetry and differential pulse voltammetry were employed to investigate the electrochemical character of the immunosensor during different modified stages using a redox probe system of K3Fe(CN)(6)/K4Fe(CN)(6). In the presence of polyclonal antibody against ractopamine, K-3[Fe(CN)(6)] electron transfer is inhibited, presumably due to that antibody in solution could adsorb on the electrode surface modified antigen. A competitive immunoreaction system was applied to determinate the free ractopamine in phosphate buffer solution. The selected polyclonal antibody showed very high sensitivity and specificity for ractopamine, and was used for the detection and quantitative determination of trace amounts of ractopamine in spiked animal feeds. (C) 2006 Elsevier B.V. All rights reserved.; Electrochemistry; SCI(E); EI; 39; ARTICLE; 4; 657-662; 9
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/251827]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Shen, Li,He, Pingli. An electrochemical immunosensor based on agarose hydrogel films for rapid determination of ractopamine[J]. 电化学通讯,2007.
APA	Shen, Li,&He, Pingli.(2007).An electrochemical immunosensor based on agarose hydrogel films for rapid determination of ractopamine.电化学通讯.
MLA	Shen, Li,et al."An electrochemical immunosensor based on agarose hydrogel films for rapid determination of ractopamine".电化学通讯 (2007).