Analysis of phospholipids by NACE with on-line ESI-MS

doi:10.1002/elps.200600533

CORC > 北京大学 > 化学与分子工程学院

	Analysis of phospholipids by NACE with on-line ESI-MS
	Gao, Fei ; Zhang, Zhengxiang ; Fu, Xiaofang ; Li, Wei ; Wang, Tao ; Liu, Huwei
刊名	electrophoresis
	2007
关键词	MS NACE phospholipids NONAQUEOUS CAPILLARY-ELECTROPHORESIS IONIZATION MASS-SPECTROMETRY PERFORMANCE LIQUID-CHROMATOGRAPHY INDIRECT ULTRAVIOLET DETECTION LASER-INDUCED FLUORESCENCE ELECTROSPRAY-IONIZATION QUANTITATIVE-ANALYSIS SIGNAL-TRANSDUCTION SEPARATION SURFACTANT
DOI	10.1002/elps.200600533
英文摘要	A hyphenated method of nonaqueous capillary electrophoresis coupled to electrospray ionization mass spectrometry (NACE-ESI-MS) is described for the simultaneous analysis of phospholipids. The best results were obtained with a mixed solution of methanol/ACN (40:60 v/v) containing 20 mM ammonium acetate and 0.5% acetic acid, under the applied voltage of 30 kV and capillary temperature of 25 degrees C. ESI-MS measurements were performed in the negative mode with methanol/ACN (40:60 v/v) containing 50 mM ammonium acetate as sheath liquid at a flow rate of 2 mu L/min. Different phospholipid classes have been successfully separated within 16 min, and the molecular species of every single class have been identified by using MS2 or MS3, which generates characteristic fragments through CID. The developed method has been applied to analyze the phospholipids extracted from rat peritoneal surface and the molecular species of phospholipid classes are presented.; Biochemical Research Methods; Chemistry, Analytical; SCI(E); 0; ARTICLE; 9; 1418-1425; 28
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/251008]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Gao, Fei,Zhang, Zhengxiang,Fu, Xiaofang,et al. Analysis of phospholipids by NACE with on-line ESI-MS[J]. electrophoresis,2007.
APA	Gao, Fei,Zhang, Zhengxiang,Fu, Xiaofang,Li, Wei,Wang, Tao,&Liu, Huwei.(2007).Analysis of phospholipids by NACE with on-line ESI-MS.electrophoresis.
MLA	Gao, Fei,et al."Analysis of phospholipids by NACE with on-line ESI-MS".electrophoresis (2007).