Aptamer biosensor for protein detection based on guanine-quenching

doi:10.1016/j.snb.2007.07.125

CORC > 北京大学 > 化学与分子工程学院

	Aptamer biosensor for protein detection based on guanine-quenching
	Wang, Wenjuan ; Chen, Chunlai ; Qian, Min Xie ; Zhao, Xin Sheng
刊名	sensors and actuators b chemical
	2008
关键词	aptamer protein detection guanine quenching thrombin biosensor aptamer beacon SINGLE-STRANDED-DNA MOLECULAR RECOGNITION SIGNALING APTAMERS ALPHA-THROMBIN RNA LIGANDS BINDING SENSORS SELECTION BEACONS D(GGTTGGTGTGGTTGG)
DOI	10.1016/j.snb.2007.07.125
英文摘要	Based on fluorescence quenching between guanine bases and fluorophore, a cost-effective biosensor for protein detection is developed. In this biosensor, the label-free protein is detected through variation in fluorescence induced by aptamer conformational change during the recognition between aptamer and protein. Using thrombin-binding aptamer and thrombin as a model system, two design strategies, which are named as hairpin design and duplex design, are developed. Moreover, we investigated how the detection limit is influenced by the modifications, the stem length, and the number of quenching guanine bases in the hairpin design, or the length of the complementary sequence in the duplex design. Our results showed that, to optimize the detection limit, the balance between binding ability of aptamer to protein and quenching efficiency of guanine quenching aptamer have to be achieved. (c) 2007 Elsevier B.V. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000253172500033&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Chemistry, Analytical; Electrochemistry; Instruments & Instrumentation; SCI(E); 28; ARTICLE; 1; 211-217; 129
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/249575]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Wang, Wenjuan,Chen, Chunlai,Qian, Min Xie,et al. Aptamer biosensor for protein detection based on guanine-quenching[J]. sensors and actuators b chemical,2008.
APA	Wang, Wenjuan,Chen, Chunlai,Qian, Min Xie,&Zhao, Xin Sheng.(2008).Aptamer biosensor for protein detection based on guanine-quenching.sensors and actuators b chemical.
MLA	Wang, Wenjuan,et al."Aptamer biosensor for protein detection based on guanine-quenching".sensors and actuators b chemical (2008).