Characterization of Column Packing Materials in High-Performance Liquid   Chromatography by Charge-Detection Quadrupole Ion Trap Mass Spectrometry

doi:10.1021/ac200966m

CORC > 北京大学 > 化学与分子工程学院

	Characterization of Column Packing Materials in High-Performance Liquid Chromatography by Charge-Detection Quadrupole Ion Trap Mass Spectrometry
	Xiong, Caiqiao ; Zhou, Xiaoyu ; Chen, Rui ; Zhang, Yiming ; Peng, Wen-Ping ; Nie, Zongxiu ; Chang, Huan-Cheng ; Liu, Huwei ; Chen, Yi
刊名	analytical chemistry
	2011
关键词	STATIONARY PHASES POLY(ETHYLENE GLYCOLS) SURFACE-AREA CALIBRATION PARTICLES CELL RETENTION DENSITY SILICA
DOI	10.1021/ac200966m
英文摘要	This article reports an application of charge-detection quadrupole ion trap mass spectrometry (CD-ITMS) to characterize the column packing materials in high-performance liquid chromatography (HPLC). Both the mean mass and the mass distribution of the packing materials are obtained and used to calculate the specific surface area of unbonded silica, the carbon load of the bonded silica, and their particle size distributions. The obtained specific surface areas and carbon loads are consistent with those measured independently by nitrogen sorption and elemental analysis respectively, whereas the derived size distributions show better resolution than that measured by a laser particle size analyzer. Furthermore, we evaluate the uniformity of particle size, which is the key parameter for column efficiency of the liquid chromatography by analyzing the mass distribution of the packing materials at the top and bottom of the column. A broader mass distribution, which yields decreased column efficiency, is observed for the column top because of the excessive use of the column. Our results suggest that CD-ITMS can serve as an alternative means for the characterization of the packing materials in HPLC and is potentially useful for column quality control.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000292280900053&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Chemistry, Analytical; SCI(E); 6; ARTICLE; 13; 5400-5406; 83
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/239414]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Xiong, Caiqiao,Zhou, Xiaoyu,Chen, Rui,et al. Characterization of Column Packing Materials in High-Performance Liquid Chromatography by Charge-Detection Quadrupole Ion Trap Mass Spectrometry[J]. analytical chemistry,2011.
APA	Xiong, Caiqiao.,Zhou, Xiaoyu.,Chen, Rui.,Zhang, Yiming.,Peng, Wen-Ping.,...&Chen, Yi.(2011).Characterization of Column Packing Materials in High-Performance Liquid Chromatography by Charge-Detection Quadrupole Ion Trap Mass Spectrometry.analytical chemistry.
MLA	Xiong, Caiqiao,et al."Characterization of Column Packing Materials in High-Performance Liquid Chromatography by Charge-Detection Quadrupole Ion Trap Mass Spectrometry".analytical chemistry (2011).