用于单分子动力学实验的微流控混合器

doi:10.3866/PKU.WHXB20110804

CORC > 北京大学 > 化学与分子工程学院

	用于单分子动力学实验的微流控混合器; A Microfluidic Mixer for Single-Molecule Kinetics Experiments
	支泽勇 ; 刘鹏程 ; 黄岩谊 ; 赵新生
刊名	物理化学学报
	2011
关键词	微流控混合单分子探测荧光共振能量传递蛋白质折叠金黄色葡萄球菌核酸酶 Microfluidic mixing Single-molecule detection Fluorescence resonance energy transfer Protein folding Staphylococcal nuclease Microfluidic mixing Single-molecule detection Fluorescence resonance energy transfer Protein folding Staphylococcal nuclease
DOI	10.3866/PKU.WHXB20110804
英文摘要	设计制作了用于单分子动力学实验的微流控混合器,该混合器用聚二甲基硅氧烷(PDMS)芯片和石英载玻片密封而成,具有低的荧光背景,广泛的生物相容性,结合激光共聚焦显微镜能够在非平衡态下进行单分子荧光探测.我们设计的压力控制系统和进样流路方便而稳定,保证了微流路中流形的长时间稳定,从而实现了样品流速和流量的精准控制.这些技术特点保证了单分子探测得到准确和高信噪比的结果.利用蛋白质的塌缩过程远快于混合过程的特点,采用荧光标记的金黄色葡萄球菌核酸酶作为指示物,分辨出蛋白质变性态的特征峰,并利用变性态的荧光共振能量传递效率随时间的变化表征出混合器在适合于单分子探测条件下的混合时间为150 ms.; We designed and built a microfluidic mixer based on the principle of hydrodynamic focusing governed by Navier-Stokes equation for single-molecule kinetics experiments. The mixer is a cast of poly(dimethylsiloxane) (PDMS) sealed with transparent fused-silica coverglass, which results in low fluorescence background and broad biological compatibility and this enables single-molecule fluorescence detection under nonequilibrium conditions. The pressure regulated sample delivery system is convenient for loading a sample and allows for precise and stable flow velocity control. The combination of microfluidic mixer and single-molecule fluorescence resonance energy transfer (smFRET) allows us to measure the time course of the distribution of the smFRET efficiency in protein folding. We used the fact that denatured protein collapses much faster than the mixing process to characterize the mixing time using donor and acceptor dyes labeled staphylococcal nuclease (SNase) as an smFRET efficiency indicator. By monitoring the smFRET efficiency of denatured SNase during the course of mixing, we determined that the mixing time was 150 ms under conditions suitable for single-molecule detection.; 国家自然科学基金; 国家重点基础研究发展规划项目(973); SCI(E); 中文核心期刊要目总览(PKU); 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 2; 8; 1990-1995; 27
语种	中文
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/238534]
专题	化学与分子工程学院工学院
推荐引用方式 GB/T 7714	支泽勇,刘鹏程,黄岩谊,等. 用于单分子动力学实验的微流控混合器, A Microfluidic Mixer for Single-Molecule Kinetics Experiments[J]. 物理化学学报,2011.
APA	支泽勇,刘鹏程,黄岩谊,&赵新生.(2011).用于单分子动力学实验的微流控混合器.物理化学学报.
MLA	支泽勇,et al."用于单分子动力学实验的微流控混合器".物理化学学报 (2011).