Temperature dependence of interaction between double stranded DNA and   Cy3 or Cy5

doi:10.1016/j.cplett.2011.08.017

CORC > 北京大学 > 化学与分子工程学院

	Temperature dependence of interaction between double stranded DNA and Cy3 or Cy5
	Li, Xun ; Yin, Yandong ; Yang, Xinxing ; Zhi, Zeyong ; Zhao, Xin Sheng
刊名	化学物理学快报
	2011
关键词	RESONANCE ENERGY-TRANSFER STRUCTURAL TOOL FRET LOCATION CHANNEL
DOI	10.1016/j.cplett.2011.08.017
英文摘要	Fluorescence resonance energy transfer (FRET) has been widely used as molecular rulers to measure the distance in biomolecules. In this Letter, double stranded DNA (dsDNA) with Cy3 and Cy5 labeled on the 5'-terminals of each strand is used to investigate how temperature influences the interaction between dsDNA and Cy3/Cy5. By applying the orientation-dependent model to fit our FRET data, we found that the interaction between dsDNA and Cy3/Cy5 is so strong that Cy3 and Cy5 are mostly capped on the end of dsDNA duplex in temperature range of 4-50 degrees C. So, the orientation factor has to be cautiously considered when using FRET to measure distance. Meanwhile, with the increase of temperature, the orientational randomization of the dyes becomes more prominent. (C) 2011 Elsevier B.V. All rights reserved.; Chemistry, Physical; Physics, Atomic, Molecular & Chemical; SCI(E); EI; 7; ARTICLE; 4-6; 271-275; 513
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/238326]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Li, Xun,Yin, Yandong,Yang, Xinxing,et al. Temperature dependence of interaction between double stranded DNA and Cy3 or Cy5[J]. 化学物理学快报,2011.
APA	Li, Xun,Yin, Yandong,Yang, Xinxing,Zhi, Zeyong,&Zhao, Xin Sheng.(2011).Temperature dependence of interaction between double stranded DNA and Cy3 or Cy5.化学物理学快报.
MLA	Li, Xun,et al."Temperature dependence of interaction between double stranded DNA and Cy3 or Cy5".化学物理学快报 (2011).