Temperature dependence of interaction between double stranded DNA and Cy3 or Cy5 | |
Li, Xun ; Yin, Yandong ; Yang, Xinxing ; Zhi, Zeyong ; Zhao, Xin Sheng | |
刊名 | 化学物理学快报
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2011 | |
关键词 | RESONANCE ENERGY-TRANSFER STRUCTURAL TOOL FRET LOCATION CHANNEL |
DOI | 10.1016/j.cplett.2011.08.017 |
英文摘要 | Fluorescence resonance energy transfer (FRET) has been widely used as molecular rulers to measure the distance in biomolecules. In this Letter, double stranded DNA (dsDNA) with Cy3 and Cy5 labeled on the 5'-terminals of each strand is used to investigate how temperature influences the interaction between dsDNA and Cy3/Cy5. By applying the orientation-dependent model to fit our FRET data, we found that the interaction between dsDNA and Cy3/Cy5 is so strong that Cy3 and Cy5 are mostly capped on the end of dsDNA duplex in temperature range of 4-50 degrees C. So, the orientation factor has to be cautiously considered when using FRET to measure distance. Meanwhile, with the increase of temperature, the orientational randomization of the dyes becomes more prominent. (C) 2011 Elsevier B.V. All rights reserved.; Chemistry, Physical; Physics, Atomic, Molecular & Chemical; SCI(E); EI; 7; ARTICLE; 4-6; 271-275; 513 |
语种 | 英语 |
内容类型 | 期刊论文 |
源URL | [http://ir.pku.edu.cn/handle/20.500.11897/238326] ![]() |
专题 | 化学与分子工程学院 |
推荐引用方式 GB/T 7714 | Li, Xun,Yin, Yandong,Yang, Xinxing,et al. Temperature dependence of interaction between double stranded DNA and Cy3 or Cy5[J]. 化学物理学快报,2011. |
APA | Li, Xun,Yin, Yandong,Yang, Xinxing,Zhi, Zeyong,&Zhao, Xin Sheng.(2011).Temperature dependence of interaction between double stranded DNA and Cy3 or Cy5.化学物理学快报. |
MLA | Li, Xun,et al."Temperature dependence of interaction between double stranded DNA and Cy3 or Cy5".化学物理学快报 (2011). |
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