Application of frontal affinity chromatography combined on-line with   mass spectrometry to screening PAI-1 inhibitors from traditional Chinese   medicine

doi:10.1039/c2ay25696g

CORC > 北京大学 > 化学与分子工程学院

	Application of frontal affinity chromatography combined on-line with mass spectrometry to screening PAI-1 inhibitors from traditional Chinese medicine
	Yuan, Xiaohong ; Hu, Daoju ; Zhu, Wei ; Xu, Xiaojie ; Lu, Chuanjian
刊名	analytical methods
	2012
关键词	PLASMINOGEN-ACTIVATOR INHIBITOR-1
DOI	10.1039/c2ay25696g
英文摘要	Frontal affinity chromatography combined on-line with mass spectrometry (FAC-MS) is an efficient method for screening traditional Chinese medicine (TCM). This paper reports the application of FAC-MS to screening PAI-1 inhibitor from TCM. Novel small molecule inhibitors derived from natural products are particularly desirable as inhibitors of PAI-1. Several promising inhibitors of PAI-1 from TCM were identified by FAC-MS. These inhibitors included chrysophanol, rhein, corilagin, and geraniin, with 50% inhibitory concentrations (IC50) of 1.06, 37.32, 30.38, and 10.48 mu M, respectively. The results indicate that FAC-MS is a direct and fast method for screening active compounds from complex molecular systems. The sample in this method is a mixture, so it is suitable for the screening Chinese herb extracts.; Chemistry, Analytical; Food Science & Technology; Spectroscopy; SCI(E); EI; 2; ARTICLE; 11; 3744-3747; 4
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/230587]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Yuan, Xiaohong,Hu, Daoju,Zhu, Wei,et al. Application of frontal affinity chromatography combined on-line with mass spectrometry to screening PAI-1 inhibitors from traditional Chinese medicine[J]. analytical methods,2012.
APA	Yuan, Xiaohong,Hu, Daoju,Zhu, Wei,Xu, Xiaojie,&Lu, Chuanjian.(2012).Application of frontal affinity chromatography combined on-line with mass spectrometry to screening PAI-1 inhibitors from traditional Chinese medicine.analytical methods.
MLA	Yuan, Xiaohong,et al."Application of frontal affinity chromatography combined on-line with mass spectrometry to screening PAI-1 inhibitors from traditional Chinese medicine".analytical methods (2012).