Complexation of DNA with poly-(L-lysine) and its copolymers in   dimethylformamide

doi:10.1016/j.polymer.2013.03.024

CORC > 北京大学 > 化学与分子工程学院

	Complexation of DNA with poly-(L-lysine) and its copolymers in dimethylformamide
	Zhou, Jihan ; Ke, Fuyou ; Xia, Yuqiong ; Sun, Jianbo ; Xu, Ning ; Li, Zi-Chen ; Liang, Dehai
刊名	polymer
	2013
关键词	Complexation Organic solvent Gene delivery POLYELECTROLYTE-SURFACTANT COMPLEXES GENE-THERAPY PROGRESS ORGANIC-SOLVENTS INTERPOLYELECTROLYTE COMPLEXES DIBLOCK COPOLYMERS NONVIRAL VECTORS PHASE-BEHAVIOR DELIVERY NANOPARTICLES PROSPECTS
DOI	10.1016/j.polymer.2013.03.024
英文摘要	The polyelectrolyte complexes formed by DNA and polycations have been widely used as non-viral vectors for gene delivery. The DNA complex prepared in aqueous solutions is usually controlled by kinetics. In this work, we demonstrated, by using salmon testes DNA (2000 bp), poly-(L-lysine) (PLL35), and poly(ethylene oxide)-b-Poly-(L-lysine) (PEO45-b-PLL35), that the DNA complexes formed in dimethylformamide (DMF) was milder than those formed in aqueous solutions. In DMF, no precipitation of DNA complex is observed even at the stoichiometric charge ratio. Taking advantage of the weak complexation and better solubility of complex in DMF, we premixed DNA and PLL at higher concentration in DMF, followed by quenching the mixture into a large amount of aqueous solution. This method alleviates the kinetic control to a large extent, and the complex obtained is smaller in size and lower in molar mass than those prepared solely in aqueous solution. The morphology of the complex, as studied by AFM, is also different. (C) 2013 Elsevier Ltd. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000318139000007&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Polymer Science; SCI(E); EI; 9; ARTICLE; 10; 2521-2527; 54
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/225144]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Zhou, Jihan,Ke, Fuyou,Xia, Yuqiong,et al. Complexation of DNA with poly-(L-lysine) and its copolymers in dimethylformamide[J]. polymer,2013.
APA	Zhou, Jihan.,Ke, Fuyou.,Xia, Yuqiong.,Sun, Jianbo.,Xu, Ning.,...&Liang, Dehai.(2013).Complexation of DNA with poly-(L-lysine) and its copolymers in dimethylformamide.polymer.
MLA	Zhou, Jihan,et al."Complexation of DNA with poly-(L-lysine) and its copolymers in dimethylformamide".polymer (2013).