The coating of smart pH-responsive polyelectrolyte brushes in capillary   and its application in CE

doi:10.1002/elps.201200518

CORC > 北京大学 > 化学与分子工程学院

	The coating of smart pH-responsive polyelectrolyte brushes in capillary and its application in CE
	Liu, Jing-Xin ; Zhao, Ming-Zhe ; Deng, Yan ; Tie, Cai ; Chen, Hong-Xu ; Zhou, Ying-Lin ; Zhang, Xin-Xiang
刊名	electrophoresis
	2013
关键词	Capillary electrophoresis Permanent coating pH-responsive Polyelectrolyte brush IONIZATION-MASS-SPECTROMETRY IONIC POLYMER LAYERS SEPARATION EFFICIENCY PROTEIN-ANALYSIS ELECTROPHORESIS MULTILAYERS STABILITY PEPTIDES FLOW
DOI	10.1002/elps.201200518
英文摘要	A novel pH-responsive coating technique was developed and applied to CE successfully in this paper. The coating was formed by bonding mixed opposite charge poly(acrylic acid) and poly(2-vinylpyridine) randomly onto the inner wall of a silica capillary. The coating processes were first characterized by ellipsometry and atomic force microscopy at macroscale and microscale, respectively. Measurements of EOF were implemented to confirm the coating. Direction and velocity of EOF became controllable from negative to positive, showing a perfect sigmoidal curve as the coating net charges alternated by the pH of BGE. The control of the EOF makes it possible to analyze different kinds of small molecules, peptides, and proteins successfully in the same capillary. Results showed that the stability and reproducibility for separations of fluoroquinolone standards were satisfactory for more than a hundred separations. A series of basic and acidic protein standards were separated with admirable efficiency and minimal adsorption using both polarities. The separation of tryptic BSA digest showed that the prepared capillary has immense potential in analyzing a single sample with both acidic and basic separations, which achieved the expectation in proteomics study by CE-MS.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000318935500012&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemical Research Methods; Chemistry, Analytical; SCI(E); 7; ARTICLE; 9-10; 1352-1358; 34
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/224183]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Liu, Jing-Xin,Zhao, Ming-Zhe,Deng, Yan,et al. The coating of smart pH-responsive polyelectrolyte brushes in capillary and its application in CE[J]. electrophoresis,2013.
APA	Liu, Jing-Xin.,Zhao, Ming-Zhe.,Deng, Yan.,Tie, Cai.,Chen, Hong-Xu.,...&Zhang, Xin-Xiang.(2013).The coating of smart pH-responsive polyelectrolyte brushes in capillary and its application in CE.electrophoresis.
MLA	Liu, Jing-Xin,et al."The coating of smart pH-responsive polyelectrolyte brushes in capillary and its application in CE".electrophoresis (2013).