以光散射技术研究啶虫脒的测定及其与DNA的相互作用

doi:10.3964/j.issn.1000-0593.2008.06.039

CORC > 北京大学 > 化学与分子工程学院

	以光散射技术研究啶虫脒的测定及其与DNA的相互作用; Study on determination of acetamiprid and interaction between acetamiprid and deoxyribonucleic acid by resonance light scattering
	杜凤沛 ; 刘刚 ; 罗小林 ; 侯士聪 ; 李克安 ; 任丽萍 ; 黄琴 ; 毛朝姝 ; 揭念芹
刊名	光谱学与光谱分析
	2008
关键词	啶虫脒共振光散射脱氧核糖核酸作用机理 acetamiprid resonance light-scattering method deoxyribonucleic acids interaction mechanism
DOI	10.3964/j.issn.1000-0593.2008.06.039
英文摘要	研究了啶虫脒与脱氧核糖核酸(DNA)之间的共振光散射(RLS)增强作用。加入啶虫脒导致DNA的共振光散射增强,在316.0 nm处,存在一处共振光散射增强峰。由此建立了一种以DNA为探针检测农药啶虫脒的新方法。体系的最佳条件为,实验选择了pH 1.73为适宜酸度;加入10μg.mL-1浓度的DNA溶液的体积为2 mL;在室温条件下,体系的反应需要30 min达到稳定;"啶虫脒-DNA-H2SO4"的加药顺序为最佳。该方法适用的线性范围为0~2.25μg.mL-1,检出限为0.2μg.mL-1,啶虫脒在河水样品中的回收率为98.0%~102.0%,并探讨了DNA与啶虫脒的相互作用机理:啶虫脒与核...; The interaction between acetamiprid and deoxyribonucleic acid (DNA) was used to determine acetamiprid by resonance light scattering (RLS). The RLS signals of DNA were greatly enhanced by acetamiprid in the spectrum region of 300-600 nm. The spectrum peak is around 316. 0 nm. The optimum conditions: pH is 1. 73; the concentration of DNA is 2. 0 mu g . mL(-1); the optimum addition order of reagent is acetamiprid -DNA-H(2)SO(4); the reaction time is 30 min; and the linear range of the calibration curve is 0-2. 25 mu g . mL(-1), with the detection limit of 0. 2 (g mu) . mL(-1). The acetamiprid in river water sample was determined. The results were satisfactory, and the recovery rates were in the range of 98%-106%. The interaction mechanism of acetamiprid and DNA was discussed: the interactions between acetamiprid and nucleic acid base include electrostatic effect and pi-pi cumulate effect.; SCI(E); EI; 中文核心期刊要目总览(PKU); 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 1; 06; 1368-1371; 28
语种	中文
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/221001]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	杜凤沛,刘刚,罗小林,等. 以光散射技术研究啶虫脒的测定及其与DNA的相互作用, Study on determination of acetamiprid and interaction between acetamiprid and deoxyribonucleic acid by resonance light scattering[J]. 光谱学与光谱分析,2008.
APA	杜凤沛.,刘刚.,罗小林.,侯士聪.,李克安.,...&揭念芹.(2008).以光散射技术研究啶虫脒的测定及其与DNA的相互作用.光谱学与光谱分析.
MLA	杜凤沛,et al."以光散射技术研究啶虫脒的测定及其与DNA的相互作用".光谱学与光谱分析 (2008).