Design, synthesis and characterization of artificial phospholipids as   model membrane receptors for specific binding of rabbit C-reactive   protein

doi:10.1016/S0968-5677(98)00128-X

CORC > 北京大学 > 化学与分子工程学院

	Design, synthesis and characterization of artificial phospholipids as model membrane receptors for specific binding of rabbit C-reactive protein
	Zhou, QZ ; Gao, QF ; Tong, J ; Zhou, Y ; Mao, J ; Sui, SF
刊名	supramolecular science
	1998
关键词	lipids monolayer C-reactive protein ellipsometry LB film COMPLEMENT-SYSTEM LIPOSOMES CONSUMPTION MONOLAYERS
DOI	10.1016/S0968-5677(98)00128-X
英文摘要	C-reactive protein (CRP) is an acute phase reactive protein, which has been shown to specifically bind to phosphorylcholine (PC) and phosphorylethanoamine (PE) moieties in the presence of calcium. In order to investigate the effect of steric hindrance on the specific binding of CRP to membranes, we designed and synthesized six phospholipids, each containing a long-arm spacer of 3, 6 or 8 atoms between the head group and hydrophobic tail. By mixing synthesized lipids and natural lipids the ligand-containing monolayers were prepared, which have PC or PE groups protruding out of the membrane surface. To characterize of the synthesized phospholipids, the thickness of the lipid monolayers was measured by surface plasmon resonance (SPR) technique, the phase behavior of the lipid monolayer at air/water interface was studied by pressure-area analysis, and the specific binding of rabbit C-reactive protein to the synthesized lipid containing membranes was studied by imaging ellipsometry. (C) 1998 Elsevier Science Limited. All rights reserved.; Chemistry, Multidisciplinary; Materials Science, Multidisciplinary; Multidisciplinary Sciences; SCI(E); EI; 1; ARTICLE; 5-6; 795-802; 5
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/215777]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Zhou, QZ,Gao, QF,Tong, J,et al. Design, synthesis and characterization of artificial phospholipids as model membrane receptors for specific binding of rabbit C-reactive protein[J]. supramolecular science,1998.
APA	Zhou, QZ,Gao, QF,Tong, J,Zhou, Y,Mao, J,&Sui, SF.(1998).Design, synthesis and characterization of artificial phospholipids as model membrane receptors for specific binding of rabbit C-reactive protein.supramolecular science.
MLA	Zhou, QZ,et al."Design, synthesis and characterization of artificial phospholipids as model membrane receptors for specific binding of rabbit C-reactive protein".supramolecular science (1998).