Mimicry of peroxidase by co-immobilization of 1-allylimidazole and hemin on N-isopropylacrylamide-based hydrogel | |
Wang, XQ ; Li, YZ ; Chang, WB | |
1999 | |
关键词 | hemin immobilization peroxidase mimicry poly-N-isopropylacrylamide IMIDAZOLE PROPYL GEL HORSERADISH-PEROXIDASE OXIDATION REACTIONS METALLOPORPHYRINS PORPHYRINS COMPLEXES POLYSTYRENE CATALYSTS OXYGEN LIGAND |
英文摘要 | Hemin and 1-allylimidazole (AI) were copolymerized covalently to N,N'-methylene-bis-acrylamide (MBA) cross-linked N-isopropylacrylamide (NIPAAm) hydrogel to form a hemin-based mimetic enzyme of peroxidase, i.e., poly(NIPAAm/MBA/hemin/imidazole). Its catalytic activity was studied in comparison with hemin and a similar hydrogel, poly(NIPAAm/MBA/hemin), that was prepared in the absence of AI. The evaluation was based on their activities in catalyzing oxidation of fluorogenic substrate p-hydroxyphenylacetic (p-HPA) with H2O2 as the oxidant. The results showed that the activity of poly(NIPAAm/MBA/hemin/imidazole) is 21% higher than that of poly(NIPAAm/MBA/hemin) and 102% higher than hemin. This mimetic enzyme can be used for the determination of hydrogen peroxide with a detection limit (S/N= 3) of 26 nmol l(-1). (C) 1999 Elsevier Science B.V. All rights reserved.; Chemistry, Analytical; SCI(E); CPCI-S(ISTP); 16 |
语种 | 英语 |
DOI标识 | 10.1016/S0003-2670(99)00625-X |
内容类型 | 其他 |
源URL | [http://ir.pku.edu.cn/handle/20.500.11897/214116] |
专题 | 化学与分子工程学院 |
推荐引用方式 GB/T 7714 | Wang, XQ,Li, YZ,Chang, WB. Mimicry of peroxidase by co-immobilization of 1-allylimidazole and hemin on N-isopropylacrylamide-based hydrogel. 1999-01-01. |
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