Complexation between DNA and peptides with precisely controlled charge   density and distribution

doi:10.1007/s10118-014-1478-x

CORC > 北京大学 > 化学与分子工程学院

	Complexation between DNA and peptides with precisely controlled charge density and distribution
	Zhou, Ji-han ; Wen, Hao ; Su, Cui-cui ; Niu, Lin ; Liang, De-hai
刊名	高分子科学英文版
	2014
关键词	Peptide Charge density DNA Polyelectrolyte complex POLYELECTROLYTE COMPLEXES LIGHT-SCATTERING PHASE-SEPARATION AQUEOUS-SOLUTION CHAIN OLIGONUCLEOTIDES TRANSFECTION RECOGNITION MICROGELS DELIVERY
DOI	10.1007/s10118-014-1478-x
英文摘要	Using three designed peptides with precisely-controlled charge density and three types of DNAs with different length and flexibility, the effect of charge density on the formation of PEC was studied. Highly charged (KKKK)(5) interacts strongly with 21 bp dsDNA to form large complex, followed by precipitation; while the medium charged (KGKG)(5) only form complex with 21 bp dsDNA at proper +/- charge ratios; and no prominent complex between weakly charged (KGGG)(5) and 21 bp dsDNA is observed at the same conditions. Similar trend is observed when the peptides form complex with 2000 bp DNA or 21nt ssDNA. It is also found that the complex formed by adding peptide to DNA is in random coil conformation, but the complex prepared by the inverse order is in molten globule state. Re-dissolution of the complex occurs only when DNA is added to peptides with similar or shorter length.; Polymer Science; SCI(E); 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 2; ARTICLE; dliang@pku.edu.cn; 11; 1460-1468; 32
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/209375]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Zhou, Ji-han,Wen, Hao,Su, Cui-cui,et al. Complexation between DNA and peptides with precisely controlled charge density and distribution[J]. 高分子科学英文版,2014.
APA	Zhou, Ji-han,Wen, Hao,Su, Cui-cui,Niu, Lin,&Liang, De-hai.(2014).Complexation between DNA and peptides with precisely controlled charge density and distribution.高分子科学英文版.
MLA	Zhou, Ji-han,et al."Complexation between DNA and peptides with precisely controlled charge density and distribution".高分子科学英文版 (2014).