Analysis of endogenous nucleotides by single cell capillary   electrophoresis-mass spectrometry

doi:10.1039/c4an01133c

CORC > 北京大学 > 化学与分子工程学院

	Analysis of endogenous nucleotides by single cell capillary electrophoresis-mass spectrometry
	Liu, Jing-Xin ; Aerts, Jordan T. ; Rubakhin, Stanislav S. ; Zhang, Xin-Xiang ; Sweedler, Jonathan V.
刊名	analyst
	2014
关键词	VOLUME SAMPLE STACKING NEGATIVE-ION FORMATION ANIONIC METABOLITES ELECTROOSMOTIC FLOW APLYSIA-CALIFORNICA MAMMALIAN-CELLS POLYMER LAYERS METABOLOMICS CHROMATOGRAPHY NEURONS
DOI	10.1039/c4an01133c
英文摘要	Analytical technologies that enable investigations at the single cell level facilitate a range of studies; here a lab-fabricated capillary electrophoresis-electrospray ionization-mass spectrometry (CE-ESI-MS) platform was used to analyze anionic metabolites from individual Aplysia califomica neurons. The system employs a customized coaxial sheath-flow nanospray interface connected to a separation capillary, with the sheath liquid and separation buffer optimized to ensure a stable spray. The method provided good repeatability of separation and reliable detection sensitivity for 16 mono:, di- and triphosphate nucleosides. For a range of anionic analytes, including cyclic adenosine monophosphate (cAMP), adenosine diphosphate (ADP) and adenosine triphosphate (ATP), the detection limits were in the low nanomolar range (<22 nM). A large Aplysia R2 neuron was used to demonstrate the ability of CE-ESI-MS to quantitatively characterize anionic metabolites within individual cells, with 15 nucleotides and derivatives detected. Following the method validation process, we probed smaller, 60 gm diameter Aplysia sensory neurons where sample stacking was used as a simple on-line analyte preconcentration approach. The calculated energy balance ([ATP] + 0.5 x [ADP1)/(EAMP] + [ADP] + [ATP]) of these cells was comparable with the value obtained from bulk samples.; Chemistry, Analytical; SCI(E); 1; ARTICLE; zxx@pku.edu.cn; jsweedle@illinois.edu; 22; 5835-5842; 139
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/207080]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Liu, Jing-Xin,Aerts, Jordan T.,Rubakhin, Stanislav S.,et al. Analysis of endogenous nucleotides by single cell capillary electrophoresis-mass spectrometry[J]. analyst,2014.
APA	Liu, Jing-Xin,Aerts, Jordan T.,Rubakhin, Stanislav S.,Zhang, Xin-Xiang,&Sweedler, Jonathan V..(2014).Analysis of endogenous nucleotides by single cell capillary electrophoresis-mass spectrometry.analyst.
MLA	Liu, Jing-Xin,et al."Analysis of endogenous nucleotides by single cell capillary electrophoresis-mass spectrometry".analyst (2014).