X-ray studies on cross-linked lysozyme crystals in acetonitrile-water mixture

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	X-ray studies on cross-linked lysozyme crystals in acetonitrile-water mixture
	Wang Z ; Zhu G ; Huang Q ; Qian M ; Shao M ; Jia Y ; Tang Y
刊名	biochimica et biophysica acta
	1998
英文摘要	Tetragonal crystals of hen egg white lysozyme were cross-linked and subjected to X-ray diffraction study in acetonitrile-water media with different acetonitrile concentrations. Crystals in neat acetonitrile did not scatter X-ray well. Structures of crystals in neat water, in 90% and 95% acetonitrile, and crystal back-soaked from acetonitrile to water, were determined to about 2 A resolution. For crystals in both 90% acetonitrile, and crystal back-soaked from acetonitrile to water, were determined to about 2 A resolution. For crystals in both 90% and 95% acetonitrile, only one protein-bond acetonitrile molecule is found in the active site cleft, and its location and binding-protein mode is similar to the C subunit of polysaccharide. The alteration in conformation and hydrogen-bond pattern involving water as solvent causes the reduction of the protein's flexibility in organic media. The back-soaked crystal regained its ordinary three-dimensional structure in water.; SCI(E); PubMed; 0; ARTICLE; 2; 335-44; 1384
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/202024]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Wang Z,Zhu G,Huang Q,et al. X-ray studies on cross-linked lysozyme crystals in acetonitrile-water mixture[J]. biochimica et biophysica acta,1998.
APA	Wang Z.,Zhu G.,Huang Q.,Qian M.,Shao M.,...&Tang Y.(1998).X-ray studies on cross-linked lysozyme crystals in acetonitrile-water mixture.biochimica et biophysica acta.
MLA	Wang Z,et al."X-ray studies on cross-linked lysozyme crystals in acetonitrile-water mixture".biochimica et biophysica acta (1998).