Optimization of post-column photolysis and electrochemical detection for   the liquid chromatographic determination of 3-nitro-L-tyrosine

doi:10.1016/S0021-9673(98)00487-7

CORC > 北京大学 > 化学与分子工程学院

	Optimization of post-column photolysis and electrochemical detection for the liquid chromatographic determination of 3-nitro-L-tyrosine
	Liu, HW ; Duda, CT ; Huang, TH ; Aruda, WO ; Kissinger, PT
刊名	journal of chromatography a
	1998
关键词	photolysis detection, LC electrochemical detection nitrotyrosine NITRIC-OXIDE AMINO-ACIDS IDENTIFICATION SUPEROXIDE PEPTIDES PROTEINS ONLINE
DOI	10.1016/S0021-9673(98)00487-7
英文摘要	A new post-column photolysis technology has been developed based on the use of a low pressure, low temperature UV lamp and TiO2 coated knitted reaction coil. As a test case the developed technique was used for the determination of 3-nitro-L-tyrosine by liquid chromatography with electrochemical detection. Different photolysis lamps and reactor tubing lengths were evaluated in terms of their effect on the separation efficiency and/or photolysis efficiency. A detection limit of 0.5 nM (10 fmol) for 3-nitro-L-tyrosine was achieved under optimized conditions, with a linear correlation coefficient of R-2=0.9898 over a concentration range of 2-100 mu M. Pre-injection photolysis of 3-nitro-L-tyrosine indicated that dihydroxyphenylalanine is the main photolysis product. In general, use of the photoreactor prior to liquid chromatography is an excellent method for exploring photodegradation products of an analyte in conjunction with the full range of available liquid chromatography detectors. (C) 1998 Elsevier Science B.V. All rights reserved.; Biochemical Research Methods; Chemistry, Analytical; SCI(E); EI; PubMed; 12; ARTICLE; 1; 69-75; 818
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/202022]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Liu, HW,Duda, CT,Huang, TH,et al. Optimization of post-column photolysis and electrochemical detection for the liquid chromatographic determination of 3-nitro-L-tyrosine[J]. journal of chromatography a,1998.
APA	Liu, HW,Duda, CT,Huang, TH,Aruda, WO,&Kissinger, PT.(1998).Optimization of post-column photolysis and electrochemical detection for the liquid chromatographic determination of 3-nitro-L-tyrosine.journal of chromatography a.
MLA	Liu, HW,et al."Optimization of post-column photolysis and electrochemical detection for the liquid chromatographic determination of 3-nitro-L-tyrosine".journal of chromatography a (1998).